In situ PCR detection of phytoplasma DNA in embryos from coconut palms with lethal yellowing disease

dc.creatorIVAN ISIDRO CORDOVA LARA
dc.creatorPhil Jones
dc.creatorNigel A. Harrison
dc.creatorCarlos Mariano Oropeza Salín
dc.date2003
dc.date.accessioned2023-07-21T19:18:47Z
dc.date.available2023-07-21T19:18:47Z
dc.identifierhttp://cicy.repositorioinstitucional.mx/jspui/handle/1003/1610
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7737170
dc.descriptionDNA of the lethal yellowing (LY) phytoplasma was detected in 13 of 72 embryos from fruits of four diseased Atlantic tall coconut palms by polymerase chain reaction (PCR) assays employing phytoplasma universal rRNA primer pair P1/P7, nested LY group‐specific rRNA primer pair 503f/LY16Sr or LY phytoplasma‐specific nonribosomal primer pair LYF1/R1. Phytoplasma distribution in sectioned tissues from six PCR positive embryos was determined by in situ PCR and digoxigenin‐11‐deoxy‐UTP (Dig) labelling of amplification products. Dig‐labeled DNA products detected by colourimetric assay were clearly evident on sections from the same three embryos investigated in detail by in situ PCRs employing primer pairs P1/P7 or LYF1/R1. Deposition of blue–green stain on sections as a result of each assay was restricted to areas of the embryos corresponding to the plumule and cells ensheathing it. By comparison, similarly treated embryo sections derived from fruits of a symptomless Atlantic tall coconut palm were consistently devoid of any stain. Presence of phytoplasma DNA in embryo tissues suggests the possible potential for seed transmission which remains to be demonstrated.
dc.formatapplication/pdf
dc.languageeng
dc.relationinfo:eu-repo/semantics/datasetDOI/https://doi.org/10.1046/j.1364-3703.2003.00152.x
dc.relationcitation:Cordova, I., Jones, P., Harrison, N. A., & Oropeza, C. (2003). In situ PCR detection of phytoplasma DNA in embryos from coconut palms with lethal yellowing disease. Molecular Plant Pathology, 4(2), 99-108.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceMolecular Plant Pathology, 4(2), 99-108, 2003.
dc.subjectinfo:eu-repo/classification/Autores/BACTERIA (MICROORGANISMS)
dc.subjectinfo:eu-repo/classification/Autores/COCOS NUCIFERA
dc.subjectinfo:eu-repo/classification/Autores/PHYTOPLASMA
dc.subjectinfo:eu-repo/classification/Autores/PROKARYOTA
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/24
dc.subjectinfo:eu-repo/classification/cti/2407
dc.subjectinfo:eu-repo/classification/cti/240702
dc.subjectinfo:eu-repo/classification/cti/240702
dc.titleIn situ PCR detection of phytoplasma DNA in embryos from coconut palms with lethal yellowing disease
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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