Use of a simple method to isolate intact RNA from partially hydrated Selaginella Lepidophylla plants

dc.creatorJUAN PABLO VALENZUELA AVENDAÑO
dc.creatorIván Alfredo Estrada Mota
dc.creatorGabriel Lizama Uc
dc.creatorRAMON ARMANDO SOUZA PERERA
dc.creatorElisa Miriam Valenzuela Soto
dc.creatorJosé Juan Zúñiga Aguilar
dc.date2005
dc.date.accessioned2023-07-21T19:18:46Z
dc.date.available2023-07-21T19:18:46Z
dc.identifierhttp://cicy.repositorioinstitucional.mx/jspui/handle/1003/1601
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7737161
dc.descriptionIsolation of high-quality RNA is a necessary step in gene expression analysis. Although many methods can be used to isolate RNA from plants where contamination of preparations with complex carbohydrates or phenolic compounds is a problem, the application of these methods toSelaginella lepidophylla tissues has failed to obtain good-quality RNA. Here we introduce 2 modifications to the method developed by Chomczynski and Sacchi (1987), generating a simple and rapid method that allows the isolation of intact RNA fromS. lepidophylla-dehydrated tissues. Although the introduced modifications are not new, their addition proved to be decisive for success in RNA isolation. Quality of the RNA obtained was evaluated by electrophoresis in agarose and by 3 different PCR-based techniques—RT-PCR, RNA differential display, and synthesis of a cDNA library.
dc.formatapplication/pdf
dc.languageeng
dc.relationinfo:eu-repo/semantics/datasetDOI/https://doi.org/10.1007/BF02772713
dc.relationcitation:Valenzuela-Avendaño, J. P., Mota, I. A. E., Uc, G. L., Perera, R. S., Valenzuela-Soto, E. M., & Aguilar, J. J. Z. (2005). Use of a simple method to isolate intact RNA from partially hydratedSelaginella lepidophylla plants. Plant Molecular Biology Reporter, 23(2), 199-200.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourcePlant Molecular Biology Reporter, 23(2), 199-200, 2005.
dc.subjectinfo:eu-repo/classification/Autores/RESURRECTION PLANTS 
dc.subjectinfo:eu-repo/classification/Autores/REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION 
dc.subjectinfo:eu-repo/classification/Autores/RNA EXTRACTION
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/24
dc.subjectinfo:eu-repo/classification/cti/2415
dc.subjectinfo:eu-repo/classification/cti/241502
dc.subjectinfo:eu-repo/classification/cti/241502
dc.titleUse of a simple method to isolate intact RNA from partially hydrated Selaginella Lepidophylla plants
dc.typeinfo:eu-repo/semantics/article


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