Molecular cloning and functional analysis of a Na + -insensitive K + transporter of Capsicum chinense Jacq

dc.creatorNANCY RUIZ LAU
dc.creatorEmanuel Bojorquez Quintal
dc.creatorBEGOÑA BENITO CASADO
dc.creatorIleana de la Caridad Echevarría Machado
dc.creatorLucila Aurelia Sánchez Cach
dc.creatorMaría de Fátima Medina Lara
dc.creatorManuel Martínez Estévez
dc.date2019
dc.date.accessioned2023-07-21T19:18:38Z
dc.date.available2023-07-21T19:18:38Z
dc.identifierhttp://cicy.repositorioinstitucional.mx/jspui/handle/1003/1540
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7737100
dc.descriptionHigh-affinity K+ (HAK) transporters are encoded by a large family of genes and are ubiquitous in the plant kingdom. These HAK-type transporters participate in low- and high-affinity potassium (K+) uptake and are crucial for the maintenance of K+ homeostasis under hostile conditions. In this study, the full-length cDNA of CcHAK1 gene was isolated from roots of the habanero pepper (Capsicum chinense). CcHAK1 expression was positively regulated by K+ starvation in roots and was not inhibited in the presence of NaCl. Phylogenetic analysis placed the CcHAK1 transporter in group I of the HAK K+ transporters, showing that it is closely related to Capsicum annuum CaHAK1 and Solanum lycopersicum LeHAK5. Characterization of the protein in a yeast mutant deficient in high-affinity K+ uptake (WΔ3) suggested that CcHAK1 function is associated with high-affinity K+ uptake, with Km and Vmax for Rb of 50 μM and 0.52 nmol mg−1 min−1, respectively. K+ uptake in yeast expressing the CcHAK1 transporter was inhibited by millimolar concentrations of the cations ammonium (NH+4) and cesium (Cs+) but not by sodium (Na+). The results presented in this study suggest that the CcHAK1 transporter may contribute to the maintenance of K+ homeostasis in root cells in C. chinense plants undergoing K+-deficiency and salt stress.
dc.formatapplication/pdf
dc.languageeng
dc.relationinfo:eu-repo/semantics/datasetDOI/10.3389/fpls.2016.01980
dc.relationcitation:Ruiz-Lau, N., Bojórquez-Quintal, E., Benito, B., Echevarría-Machado, I., Sánchez-Cach, L. A., Medina-Lara, M. D. F., & Martínez-Estévez, M. (2016). Molecular cloning and functional analysis of a Na+-insensitive K+ transporter of Capsicum chinense Jacq. Frontiers in plant science, 7, 1980.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceFrontiers in plant science, 7, 1980.
dc.subjectinfo:eu-repo/classification/Autores/CAPSICUM CHINENSE
dc.subjectinfo:eu-repo/classification/Autores/HAK-TYPE TRANSPORTER
dc.subjectinfo:eu-repo/classification/Autores/K+-STARVATION
dc.subjectinfo:eu-repo/classification/Autores/POTASSIUM
dc.subjectinfo:eu-repo/classification/Autores/ROOTS
dc.subjectinfo:eu-repo/classification/Autores/SODIUM
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/2
dc.titleMolecular cloning and functional analysis of a Na + -insensitive K + transporter of Capsicum chinense Jacq
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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