Un protocolo de embriogénesis somática para la regeneración y caracterización in vitro de Laelia anceps ssp. dawsonii

A protocol of somatic embryogenesis for the in vitroregeneration and characterization of Laelia anceps ssp. dawsonii

dc.creatorHilda Eulalia Lee Espinosa
dc.creatorANTONIO LAGUNA CERDA
dc.creatorJoaquín Murguía González
dc.creatorLourdes Georgina Iglesias Andreu
dc.creatorBENJAMIN GARCIA ROSAS
dc.creatorDIANA ESCOBEDO LOPEZ
dc.creatorYOLANDA MARIA MARTINEZ OCAMPO
dc.creatorFELIPE ALONSO BARREDO POOL
dc.creatorNANCY SANTANA BUZZY
dc.date2010
dc.date.accessioned2023-07-21T19:18:25Z
dc.date.available2023-07-21T19:18:25Z
dc.identifierhttp://cicy.repositorioinstitucional.mx/jspui/handle/1003/1461
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7737024
dc.descriptionLaelia anceps ssp. dawsonii is one of the most appreciated Mexican wild orchids, with atractive characteristics for ornamental potential according to international standards. It has become an important economic species, subjected to excessive collection and risk of extinction. To ensure its conservation, it is necessary to develop efficient propagation protocols which allow its sustainable utilization and reduce collection. Somatic embryogenesis is an efficient technology for in vitro plant multiplication. In this study we evaluated in vitro conditions for developing a somatic embryogenesis protocol for Laelia anceps ssp. dawsonii. Embryogenic calli was induced from mature seeds, in a Murashige and Skoog medium supplemented with multiple combinations of naphthalene acetic acid (NAA), 6-benzylaminopurine (BAP), kinetin (Kin) and indole 3 acetic acid (IAA). The combination NAA+BAP+IAA (2.0 mg L-1 each) was optimal for callus induction, producing 611 embryoids in a 16 h photoperiod (33.8 µmol m-2 s-1) when subcultured every 45 d on the same medium. This conditions allowed for complete plantlet development. In approximately three months, plantlets achieved 4-5 cm in a V&W medium supplemented with BAP (2 mg L-1), AIA (1 mg L-1) and 0.2 % activated charcoal; after 30 d plantlets were acclimatized in a greenhouse with 95 % survival rate.
dc.formatapplication/pdf
dc.languagespa
dc.relationcitation:Lee Espinosa, H. E., Laguna Cerda, A., Murguia Gonzalez, J., Iglesias-Andreu, L., Garcia Rosas, B., Escobedo Lopez, D., ... & Santana Buzzy, N. (2010). A protocol of somatic embryogenesis for the in vitro regeneration and characterization of Laelia anceps ssp. Dawsonii. Revista Fitotecnia Mexicana, 33(4), 323-332.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceRevista Fitotecnia Mexicana, 33(4), 323-332, 2010
dc.subjectinfo:eu-repo/classification/Autores/LAELIA ANCEPS SSP. DAWSONII
dc.subjectinfo:eu-repo/classification/Autores/IN VITRO CULTURE
dc.subjectinfo:eu-repo/classification/Autores/SOMATIC EMBRYOGENESIS
dc.subjectinfo:eu-repo/classification/Autores/ORCHIDACEAE
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/2
dc.titleUn protocolo de embriogénesis somática para la regeneración y caracterización in vitro de Laelia anceps ssp. dawsonii
dc.titleA protocol of somatic embryogenesis for the in vitroregeneration and characterization of Laelia anceps ssp. dawsonii
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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