Cloning, in silico structural characterization and expression analysis of MfAtr4, an ABC transporter from the banana pathogen Mycosphaerella fijiensis

dc.creatorYENY LIZZET COUOH UICAB
dc.creatorIGNACIO RODRIGO ISLAS FLORES
dc.creatorNUVIA EUGENIA KANTUN MORENO
dc.creatorMIGUEL ALONSO TZEC SIMA
dc.creatorSANTY PERAZA ECHEVERRIA
dc.creatorLIGIA GUADALUPE BRITO ARGAEZ
dc.creatorLETICIA PERAZA ECHEVERRIA
dc.creatorROSA GRIJALVA ARANGO
dc.creatorANDREW CHRISTOPHER JAMES
dc.creatorCECILIA MONICA RODRIGUEZ GARCIA
dc.date2012
dc.date.accessioned2023-07-21T19:17:43Z
dc.date.available2023-07-21T19:17:43Z
dc.identifierhttp://cicy.repositorioinstitucional.mx/jspui/handle/1003/1048
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7736633
dc.descriptionABC transporters are membrane proteins that use the energy released from the hydrolysis of ATP to drive the transport of compounds across biological membranes. In some plants, pathogenic fungi ABC transporters play a role as virulence factors by mediating the export of plant defense compounds or fungal virulence factors. Mycosphaerella fijiensis, the causal agent of black Sigatoka disease in banana, is the main constraint for the banana industry worldwide. So far, little is known about molecular mechanism that it uses to infect the host. In this study, degenerated primers designed from fungal ABC transporters known to be involved in virulence were used to isolate homologs from M. fijiensis.Here, we reported the full cloning of MfAtr4 a putative ortholog of MgAtr4, an ABC transporter of the related Mycosphaerella graminicola with a function in virulence. Similarities and differences with its presumed ortholog MgAtr4 are described, and the putative function of MfAtr4 are discussed. Analysis of MfAtr4 gene expression in field banana samples exhibiting visible symptoms of black Sigatoka disease indicated a higher expression of MfAtr4 during the first symptomatic stages in comparison to the late necrotrophic phases, suggesting a role for MfAtr4 in the early stages of pathogenic development of M. fijiensis.
dc.formatapplication/pdf
dc.languageeng
dc.relationinfo:eu-repo/semantics/datasetDOI/DOI: 10.5897/AJB11.2966
dc.relationcitation:Couoh-Uicab, Y., Islas-Flores, L., Kantún-Moreno, N., Zwiers, L. H., Tzec-Simá, M., Peraza-Echeverría, S., ... & Rodríguez-García, C. (2012). Cloning, in silico structural characterization and expression analysis of MfAtr4, an ABC transporter from the banana pathogen Mycosphaerella fijiensis. African Journal of Biotechnology, 11(1), 54-79.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.source African Journal of Biotechnology, 11(1), 54-79, 2012
dc.subjectinfo:eu-repo/classification/Autores/ABC TRANSPORTERS
dc.subjectinfo:eu-repo/classification/Autores/VIRULENCE FACTORS
dc.subjectinfo:eu-repo/classification/Autores/MGATR4 ORTHOLOG
dc.subjectinfo:eu-repo/classification/Autores/MYCOSPHAERELLA FIJIENSIS
dc.subjectinfo:eu-repo/classification/Autores/BLACK SIGATOKA
dc.subjectinfo:eu-repo/classification/Autores/MUSA SP.
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/2
dc.titleCloning, in silico structural characterization and expression analysis of MfAtr4, an ABC transporter from the banana pathogen Mycosphaerella fijiensis
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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