Direct colony polymerase chain reaction (PCR): An efficient technique to rapidly identify and distinguish Mycosphaerella fijiensis and Mycosphaerella musicola

dc.creatorROBERTO CARLOS VAZQUEZ EUAN
dc.creatorROSA GRIJALVA ARANGO
dc.creatorBARTOLOME HUMBERTO CHI MANZANERO
dc.creatorMIGUEL ALONSO TZEC SIMA
dc.creatorIGNACIO RODRIGO ISLAS FLORES
dc.creatorCECILIA MONICA RODRIGUEZ GARCIA
dc.creatorLETICIA PERAZA ECHEVERRIA
dc.creatorANDREW CHRISTOPHER JAMES
dc.creatorGILBERTO MANZO SANCHEZ
dc.creatorBLONDY BEATRIZ CANTO CANCHE
dc.date2012
dc.date.accessioned2023-07-21T19:17:43Z
dc.date.available2023-07-21T19:17:43Z
dc.identifierhttp://cicy.repositorioinstitucional.mx/jspui/handle/1003/1047
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7736632
dc.descriptionSigatoka disease is the most important threat for banana production worldwide. Many species of Mycosphaerella have been described from banana but, to date, the three species Mycosphaerella fijiensis, M. musicola and M. eumusae are the only species found to be pathogenic to banana. Reliable identification by classical methods requires expertise because these fungi produce similar symptoms and they are morphologically similar. For studies of ecology, genetic diversity and epidemiology their differentiation is crucial. Several laboratories have developed molecular protocols to differentiate these fungi. Currently, a number of primers targeting ribosomal sequences, actin, tubulin and histone 3 genes are available for diagnosis of the Sigatoka complex. In the present work, we report a direct colony-polymerase chain reaction (DC-PCR) approach to rapidly distinguish M. fijiensis and M. musicola strains in multiplex PCR reactions. This is the most economical and the fastest procedure reported so far for diagnosis of these two Mycosphaerella species, which are distributed in banana-growing regions in the world; the DC-PCR technique was also found to be amenable for the identification of mating type of M. fijiensis isolates. This DC-PCR may also be applicable to prepare DNA templates for basic PCR-based analyses in other fungi.
dc.formatapplication/pdf
dc.languageeng
dc.relationinfo:eu-repo/semantics/datasetDOI/DOI: 10.5897/AJB11.4044
dc.relationcitation:Vázquez-Euán, R., Grijalva-Arango, R., Chi-Manzanero, B., Tzec-Simá, M., Islas-Flores, I., Rodríguez-García, C., ... & Canto-Canché, B. (2012). Direct colony polymerase chain reaction (PCR): An efficient technique to rapidly identify and distinguish Mycosphaerella fijiensis and Mycosphaerella musicola. African Journal of Biotechnology, 11(33), 8172-8180.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceAfrican Journal of Biotechnology, 11(33), 8172-8180, 2012
dc.subjectinfo:eu-repo/classification/Autores/SIGATOKA DISEASES
dc.subjectinfo:eu-repo/classification/Autores/BANANA’S MYCOSPHAERELLA
dc.subjectinfo:eu-repo/classification/Autores/MOLECULAR DIAGNOSIS
dc.subjectinfo:eu-repo/classification/Autores/DIRECT COLONY PCR
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/2
dc.titleDirect colony polymerase chain reaction (PCR): An efficient technique to rapidly identify and distinguish Mycosphaerella fijiensis and Mycosphaerella musicola
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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