Construction and characterization of a partial binary bacterial artificial chromosome (BIBAC) of Agave tequilana var. azul (2X) and its application for gene identification

dc.creatorMARIA CONCEPCION TAMAYO ORDOÑEZ
dc.creatorLUIS CARLOS RODRIGUEZ ZAPATA
dc.creatorLORENZO FELIPE SANCHEZ TEYER
dc.date2012
dc.date.accessioned2023-07-21T19:17:09Z
dc.date.available2023-07-21T19:17:09Z
dc.identifierhttp://cicy.repositorioinstitucional.mx/jspui/handle/1003/732
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7736305
dc.descriptionThe structure and organization of the genome of Agave is still unknown. To provide a genomic tool for searching sequences of the genus, we built and characterized a binary (BIBAC2) genomic library of Agave tequilana  Weber var. azul. Clones of the library had an average insert size of 170 Kb. The frequency of inserts with  internal Not I sites was 30% and only 5% of the library showed organelle contamination. The library was  assessed using probes with high homology to repeated regions (retroelements and rDNA regions), genes  involved in the resistance to diseases (NBS-LRR) and genes related to late embryogenesis (LEA).  Recombinant clones that hybridized with each of the probes were identified. Our results indicate that the  obtained genomic library is suitable for the identification of sequences of interest, for genetic mapping and for studies of gene regulation and expression.
dc.formatapplication/pdf
dc.languageeng
dc.relationinfo:eu-repo/semantics/datasetDOI/DOI: 10.5897/AJB12.2041
dc.relationcitation:Tamayo-Ordoñez, M., Rodríguez-Zapata, L. C., & Sánchez-Teyer, L. F. (2012). Construction and characterization of a partial binary bacterial artificial chromosome (BIBAC) of Agave tequilana var. azul (2X) and its application for gene identification. African Journal of Biotechnology, 11(93), 15950-15958.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceAfrican Journal of Biotechnology, 11(93), 15950-15958, 2012
dc.subjectinfo:eu-repo/classification/Autores/BINARY BACTERIAL ARTIFICIAL CHROMOSOME (BIBAC)
dc.subjectinfo:eu-repo/classification/Autores/AGAVE TEQUILANA
dc.subjectinfo:eu-repo/classification/Autores/GENOME
dc.subjectinfo:eu-repo/classification/Autores/CLONES
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/2
dc.titleConstruction and characterization of a partial binary bacterial artificial chromosome (BIBAC) of Agave tequilana var. azul (2X) and its application for gene identification
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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