| dc.creator | Elder de la Rosa Cruz | |
| dc.date | 2008-01-31 | |
| dc.date.accessioned | 2023-07-21T15:15:46Z | |
| dc.date.available | 2023-07-21T15:15:46Z | |
| dc.identifier | http://cio.repositorioinstitucional.mx/jspui/handle/1002/1140 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/7725902 | |
| dc.description | Advances in plasmonic nanoparticle synthesis afford new opportunities for biosensing applications. Here, we apply a combination of a new type of plasmonic nanomaterial – stellated nanoparticles, and polarization-sensitive darkfield microscopy for detecting molecular assemblies and tracking of individual epidermal growth factor receptors within single live cells with high signal-to-background ratio. Depolarization of linear polarized light by stellated nanoparticles is over 15-fold more efficient than similarly-sized spheroidal nanoparticles. This efficient light depolarization allows robust detection of molecules labeled with stellated nanoparticles in cross-polarized imaging where the intrinsic light scattering from cells is significantly reduced. The imaging can be carried out with single molecule sensitivity for essentially unlimited time with no signal degradation. | |
| dc.format | application/pdf | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.rights | http://creativecommons.org/licenses/by-nc-nd/4.0 | |
| dc.subject | info:eu-repo/classification/cti/1 | |
| dc.subject | info:eu-repo/classification/cti/1 | |
| dc.title | Polarization microscopy with stellated gold nanoparticles for robust, in-situ monitoring of biomolecules | |
| dc.type | info:eu-repo/semantics/article | |
