Changes in genome-wide gene expression during allopolyploidization and genome stabilizationin hexaploid wheat

dc.creatorYeonju Jung
dc.creatorKanako Kawaura
dc.creatorKohei Mishina
dc.creatorShun Sakuma
dc.creatorMasahiro, K.
dc.creatorOgihara, Y.
dc.date2023-07-14T00:20:15Z
dc.date2023-07-14T00:20:15Z
dc.date2014
dc.date.accessioned2023-07-17T20:10:42Z
dc.date.available2023-07-17T20:10:42Z
dc.identifierhttps://hdl.handle.net/10883/22642
dc.identifier10.1266/ggs.89.215
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7514384
dc.descriptionAllopolyploidization is an important evolutionary event in plants, but its genome-wide effects are not fully understood. Common wheat, Triticum aestivum(AABBDD), evolved through amphidiploidization between T. turgidum (AABB) and Aegilops tauschii (DD). Here, global gene expression patterns in the seedlings of a synthetic triploid wheat line (ABD), its chromosome-doubled hexaploid (AABBDD) and stable synthetic hexaploid (AABBDD), and the parental lines T. turgidum (AABB) and Ae. tauschii (DD) were compared using an oligo-DNA microarray to identify metabolic pathways affected by the genome conflict that occurs during allopolyploidization and genome stabilization. Characteristic gene expression patterns of non-additively expressed genes were detected in the newly synthesized triploid and hexaploid, and in the stable synthetic hexaploid. Hierarchical clustering of all differentially expressed and non-additively expressed genes revealed that the gene expression patterns of the triploid (ABD) were similar to those of the maternal parent (AABB), and that expression patterns in successive generations arising from self-pollination became closer to that of the pollen parent (DD). The non-additive gene expression profiles markedly differed between the triploid (ABD) and chromosome-doubled hexaploid (AABBDD), as supported by Gene Ontology (GOSlim) analysis. Four hundred and nineteen non-additively expressed genes were commonly detected in all three generations. GOSlim analysis indicated that these non-additively expressed genes were predominantly involved in “biological pathways”. Notably, four of 11 genes related to sugar metabolism displayed elevated expression throughout allopolyploidization. These may be useful candidates for promoting heterosis and adaptation in plants.
dc.description215-225
dc.languageEnglish
dc.publisherGenetics Society of Japan
dc.rightsCIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose
dc.rightsOpen Access
dc.source5
dc.source89
dc.source1341-7568
dc.sourceGenes and Genetic Systems
dc.subjectAGRICULTURAL SCIENCES AND BIOTECHNOLOGY
dc.subjectAllopolyploidization
dc.subjectDNA Microarray
dc.subjectHexaploid Wheat
dc.subjectDNA
dc.subjectGENE EXPRESSION
dc.subjectHETEROSIS
dc.subjectHEXAPLOIDY
dc.subjectWHEAT
dc.subjectWheat
dc.titleChanges in genome-wide gene expression during allopolyploidization and genome stabilizationin hexaploid wheat
dc.typeArticle
dc.typePublished Version
dc.coverageJapan


Este ítem pertenece a la siguiente institución