DIW1 encoding a clade I PP2C phosphatase negatively regulates drought tolerance by de-phosphorylating TaSnRK1.1 in wheat

dc.creatorJingyi Wang
dc.creatorChaonan Li
dc.creatorLong Li
dc.creatorGao, L.
dc.creatorGe Hu
dc.creatorYanfei Zhang
dc.creatorReynolds, M.P.
dc.creatorXueyong Zhang
dc.creatorJizeng Jia
dc.creatorXinguo Mao
dc.creatorRuilian Jing
dc.date2023-07-05T20:20:13Z
dc.date2023-07-05T20:20:13Z
dc.date2023
dc.date.accessioned2023-07-17T20:10:42Z
dc.date.available2023-07-17T20:10:42Z
dc.identifierhttps://hdl.handle.net/10883/22638
dc.identifier10.1111/jipb.13504
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7514380
dc.descriptionDrought seriously impacts wheat production (Triticum aestivum L.), while the exploitation and utilization of genes for drought tolerance are insufficient. Leaf wilting is a direct reflection of drought tolerance in plants. Clade A PP2Cs are abscisic acid (ABA) co-receptors playing vital roles in the ABA signaling pathway, regulating drought response. However, the roles of other clade PP2Cs in drought tolerance, especially in wheat, remain largely unknown. Here, we identified a gain-of-function drought-induced wilting 1 (DIW1) gene from the wheat Aikang 58 mutant library by map-based cloning, which encodes a clade I protein phosphatase 2C (TaPP2C158) with enhanced protein phosphatase activity. Phenotypic analysis of overexpression and CRISPR/Cas9 mutant lines demonstrated that DIW1/TaPP2C158 is a negative regulator responsible for drought resistance. We found that TaPP2C158 directly interacts with TaSnRK1.1 and de-phosphorylates it, thus inactivating the TaSnRK1.1–TaAREB3 pathway. TaPP2C158 protein phosphatase activity is negatively correlated with ABA signaling. Association analysis suggested that C-terminal variation of TaPP2C158 changing protein phosphatase activity is highly correlated with the canopy temperature, and seedling survival rate under drought stress. Our data suggest that the favorable allele with lower phosphatase activity of TaPP2C158 has been positively selected in Chinese breeding history. This work benefits us in understanding the molecular mechanism of wheat drought tolerance, and provides elite genetic resources and molecular markers for improving wheat drought tolerance.
dc.languageEnglish
dc.publisherWiley-Blackwell Publishing Ltd
dc.rightsCIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose
dc.rightsOpen Access
dc.sourceIn press
dc.source1672-9072
dc.sourceJournal of Integrative Plant Biology
dc.subjectAGRICULTURAL SCIENCES AND BIOTECHNOLOGY
dc.subjectAssociation Analysis
dc.subjectElite Genetic Resources
dc.subjectMap‐Based Clones
dc.subjectProtein Phosphatase 2C
dc.subjectDROUGHT
dc.subjectGENETIC RESOURCES
dc.subjectPROTEINS
dc.subjectWHEAT
dc.subjectWILTING
dc.subjectWheat
dc.titleDIW1 encoding a clade I PP2C phosphatase negatively regulates drought tolerance by de-phosphorylating TaSnRK1.1 in wheat
dc.typeArticle
dc.typePublished Version
dc.coverageUnited Kingdom


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