Relocation of Sr48 to chromosome 2D using an alternative mapping population and development of a closely linked marker using diverse molecular technologies

dc.creatorNsabiyera, V.
dc.creatorQureshi, N.
dc.creatorJianbo Li
dc.creatorRandhawa, M.S.
dc.creatorPeng Zhang
dc.creatorForrest, K.L.
dc.creatorBansal, U.
dc.creatorBariana, H.S.
dc.date2023-06-22T20:20:12Z
dc.date2023-06-22T20:20:12Z
dc.date2023
dc.date.accessioned2023-07-17T20:10:38Z
dc.date.available2023-07-17T20:10:38Z
dc.identifierhttps://hdl.handle.net/10883/22624
dc.identifier10.3390/plants12081601
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7514367
dc.descriptionThe Ug99-effective stem rust resistance gene Sr48 was mapped to chromosome 2A based on its repulsion linkage with Yr1 in an Arina/Forno recombinant inbred line (RIL) population. Attempts to identify markers closely linked to Sr48 using available genomic resources were futile. This study used an Arina/Cezanne F5:7 RIL population to identify markers closely linked with Sr48. Using the Arina/Cezanne DArTseq map, Sr48 was mapped on the short arm of chromosome 2D and it co-segregated with 12 markers. These DArTseq marker sequences were used for BlastN search to identify corresponding wheat chromosome survey sequence (CSS) contigs, and PCR-based markers were developed. Two simple sequence repeat (SSR) markers, sun590 and sun592, and two Kompetitive Allele-Specific PCR (KASP) markers were derived from the contig 2DS_5324961 that mapped distal to Sr48. Molecular cytogenetic analysis using sequential fluorescent in situ hybridization (FISH) and genomic in situ hybridization (GISH) identified a terminal translocation of chromosome 2A in chromosome 2DL of Forno. This translocation would have led to the formation of a quadrivalent involving chromosomes 2A and 2D in the Arina/Forno population, which would have exhibited pseudo-linkage between Sr48 and Yr1 in chromosome 2AL. Polymorphism of the closet marker sunKASP_239 among a set of 178 wheat genotypes suggested that this marker can be used for marker-assisted selection of Sr48.
dc.languageEnglish
dc.publisherMDPI
dc.relationhttps://www.mdpi.com/article/10.3390/plants12081601/s1
dc.rightsCIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose
dc.rightsOpen Access
dc.source8
dc.source12
dc.source2223-7747
dc.sourcePlants
dc.source1601
dc.subjectAGRICULTURAL SCIENCES AND BIOTECHNOLOGY
dc.subjectFluorescent in Situ Hybridization
dc.subjectGenomic in Situ Hybridization
dc.subjectUg99
dc.subjectMARKER-ASSISTED SELECTION
dc.subjectSTEM RUST
dc.subjectTRANSLOCATION
dc.subjectWHEAT
dc.subjectWheat
dc.titleRelocation of Sr48 to chromosome 2D using an alternative mapping population and development of a closely linked marker using diverse molecular technologies
dc.typeArticle
dc.typePublished Version
dc.coverageBasel (Switzerland)


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