An efficient transformation method for genome editing of elite bread wheat cultivars

dc.creatorBiswal, A.K.
dc.creatorHernandez, L.R.B.
dc.creatorCastillo, A.I.R.
dc.creatorDebernardi, J.M.
dc.creatorDhugga, K.
dc.date2023-06-22T20:10:11Z
dc.date2023-06-22T20:10:11Z
dc.date2023
dc.date.accessioned2023-07-17T20:10:37Z
dc.date.available2023-07-17T20:10:37Z
dc.identifierhttps://hdl.handle.net/10883/22616
dc.identifier10.3389/fpls.2023.1135047
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7514359
dc.descriptionAn efficient genetic transformation protocol is necessary to edit genes for trait improvement directly in elite bread wheat cultivars. We used a protein fusion between a wheat growth-regulating factor 4 (GRF4) and its interacting factor (GIF1) to develop a reproducible genetic transformation and regeneration protocol, which we then used to successfully transform elite bread wheat cultivars Baj, Kachu, Morocco, Reedling, RL6077, and Sujata in addition to the experimental cultivar Fielder. Immature embryos were transformed with the vector using particle bombardment method. Transformation frequency increased nearly 60-fold with the GRF4-GIF1-containing vectors as compared to the control vector and ranged from ~5% in the cultivar Kachu to 13% in the cultivar RL6077. We then edited two genes that confer resistance against leaf rust and powdery mildew directly in the aforementioned elite cultivars. A wheat promoter, TaU3 or TaU6, to drive the expression of guide RNA was effective in gene editing whereas the OsU3 promoter failed to generate any edits. Editing efficiency was nearly perfect with the wheat promoters. Our protocol has made it possible to edit genes directly in elite wheat cultivars and would be useful for gene editing in other wheat varieties, which have been recalcitrant to transformation thus far.
dc.languageEnglish
dc.publisherFrontiers Media S.A.
dc.relationhttps://figshare.com/collections/An_efficient_transformation_method_for_genome_editing_of_elite_bread_wheat_cultivars/6648563
dc.rightsCIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose
dc.rightsOpen Access
dc.source14
dc.source1664-462X
dc.sourceFrontiers in Plant Science
dc.source1135047
dc.subjectAGRICULTURAL SCIENCES AND BIOTECHNOLOGY
dc.subjectParticle Bombardment
dc.subjectWheat Growth-Regulating Factor 4
dc.subjectWheat Growth-Interacting Factor
dc.subjectWheat Mildew Locus O
dc.subjectCRISPR
dc.subjectGENE EDITING
dc.subjectTRANSFORMATION
dc.subjectWHEAT
dc.subjectGenetic Resources
dc.titleAn efficient transformation method for genome editing of elite bread wheat cultivars
dc.typeArticle
dc.typePublished Version
dc.coverageSwitzerland


Este ítem pertenece a la siguiente institución