Multi-omic characterization of the maize GPI synthesis mutant gwt1 with defects in kernel development

dc.creatorRunmiao Tian
dc.creatorJianjun Jiang
dc.creatorShirong Bo
dc.creatorHui Zhang
dc.creatorXuehai Zhang
dc.creatorHearne, S.
dc.creatorJihua Tang
dc.creatorDong Ding
dc.creatorZhiyuan Fu
dc.date2023-04-28T00:10:16Z
dc.date2023-04-28T00:10:16Z
dc.date2023
dc.date.accessioned2023-07-17T20:10:33Z
dc.date.available2023-07-17T20:10:33Z
dc.identifierhttps://hdl.handle.net/10883/22580
dc.identifier10.1186/s12870-023-04188-w
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7514323
dc.descriptionBackground: Glycosylphosphatidylinositol (GPI) and GPI-anchored proteins (GAPs) are important for cell wall formation and reproductive development in Arabidopsis. However, monocot counterparts that function in kernel endosperm development have yet to be discovered. Here, we performed a multi-omic analysis to explore the function of GPI related genes on kernel development in maize. Results: In maize, 48 counterparts of human GPI synthesis and lipid remodeling genes were identified, in which null mutation of the glucosaminyl-phosphatidylinositol O-acyltransferase1 gene, ZmGWT1, caused a kernel mutant (named gwt1) with defects in the basal endosperm transport layer (BETL). We performed plasma membrane (PM) proteomics to characterize the potential GAPs involved in kernel development. In total, 4,981 proteins were successfully identified in 10-DAP gwt1 kernels of mutant and wild-type (WT), including 1,638 membrane-anchored proteins with different posttranslational modifications. Forty-seven of the 256 predicted GAPs were differentially accumulated between gwt1 and WT. Two predicted BETL-specific GAPs (Zm00001d018837 and Zm00001d049834), which kept similar abundance at general proteome but with significantly decreased abundance at membrane proteome in gwt1 were highlighted. Conclusions: Our results show the importance of GPI and GAPs for endosperm development and provide candidate genes for further investigation of the regulatory network in which ZmGWT1 participates.
dc.description191
dc.languageEnglish
dc.publisherBioMed Central
dc.relationhttps://figshare.com/articles/dataset/Additional_file_1_of_Multi-omic_characterization_of_the_maize_GPI_synthesis_mutant_gwt1_with_defects_in_kernel_development/22620238
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dc.rightsOpen Access
dc.source1
dc.source23
dc.source1471-2229
dc.sourceBMC Plant Biology
dc.subjectAGRICULTURAL SCIENCES AND BIOTECHNOLOGY
dc.subjectMaize Endosperm Development
dc.subjectMembrane Proteomics
dc.subjectGlycosyl-Phosphatidyl-Inositol
dc.subjectMembrane Anchored Proteins
dc.subjectMAIZE
dc.subjectENDOSPERM
dc.subjectMEMBRANES
dc.subjectPROTEOMICS
dc.subjectTRANSCRIPTOMICS
dc.subjectGenetic Resources
dc.titleMulti-omic characterization of the maize GPI synthesis mutant gwt1 with defects in kernel development
dc.typeArticle
dc.typePublished Version
dc.coverageLondon (United Kingdom)


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