Effect of F1 and F2 generations on genetic variability and working steps of doubled haploid production in maize

dc.creatorDe Oliveira Couto, E.G.
dc.creatorCury, M.N.
dc.creatorBandeira e Sousa, M.
dc.creatorGranato, I.
dc.creatorVidotti, M.S.
dc.creatorGarbuglio, D.D.
dc.creatorCrossa, J.
dc.creatorBurgueño, J.
dc.creatorFritsche-Neto, R.
dc.date2023-02-10T19:08:00Z
dc.date2023-02-10T19:08:00Z
dc.date2019
dc.date.accessioned2023-07-17T20:10:21Z
dc.date.available2023-07-17T20:10:21Z
dc.identifierhttps://hdl.handle.net/10883/22510
dc.identifier10.1371/journal.pone.0224631
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7514253
dc.descriptionFor doubled haploid (DH) production in maize, F1 generation has been the most frequently used for haploid induction due to facility in the process. However, using F2 generation would be a good alternative to increase genetic variability owing to the additional recombination in meiosis. Our goals were to compare the effect of F1 and F2 generations on DH production in tropical germplasm, evaluating the R1-navajo expression in seeds, the working steps of the methodology, and the genetic variability of the DH lines obtained. Sources germplasm in F1 and F2 generations were crossed with the tropicalized haploid inducer LI-ESALQ. After harvest, for both induction crosses were calculated the haploid induction rate (HIR), diploid seed rate (DSR), and inhibition seed rate (ISR) using the total number of seeds obtained. In order to study the effectiveness of the DH working steps in each generation, the percentage per se and the relative percentage were verified. In addition, SNP markers were obtained for genetic variability studies. Results showed that the values for HIR, ISR, and DSR were 1.23%, 23.48%, and 75.21% for F1 and 1.78%, 15.82%, and 82.38% for F2, respectively. The effectiveness of the DH working step showed the same percentage per se value (0.4%) for F1 and F2, while the relative percentage was 27.2% for F1 and 22.4% for F2. Estimates of population parameters in DH lines from F1 were higher than F2. Furthermore, population structure and kinship analyses showed that one additional generation was not sufficient to create new genotype subgroups. Additionally, the relative efficiency of the response to selection in the F1 was 31.88% higher than F2 due to the number of cycles that are used to obtain the DH. Our results showed that in tropical maize, the use of F1 generation is recommended due to a superior balance between time and genetic variability.
dc.languageEnglish
dc.publisherPublic Library of Science
dc.rightsCIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose
dc.rightsOpen Access
dc.source11
dc.source14
dc.source0224631
dc.source1932-6203
dc.sourcePLoS ONE
dc.subjectAGRICULTURAL SCIENCES AND BIOTECHNOLOGY
dc.subjectDoubled Haploid
dc.subjectTropical Germplasm
dc.subjectGenetic Variability
dc.subjectDIPLOIDY
dc.subjectGENETIC MARKERS
dc.subjectGENETIC VARIATION
dc.subjectGERMPLASM
dc.subjectMAIZE
dc.subjectPOPULATION STRUCTURE
dc.subjectSINGLE NUCLEOTIDE POLYMORPHISM
dc.subjectPLANT BREEDING
dc.subjectGenetic Resources
dc.titleEffect of F1 and F2 generations on genetic variability and working steps of doubled haploid production in maize
dc.typeArticle
dc.typePublished Version
dc.coverageSan Francisco, CA (USA)


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