Genome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars

dc.creatorMuhammad Ibrar Khan
dc.creatorKainat, Z.
dc.creatorMaqbool, S.
dc.creatorMehwish, A.
dc.creatorAhmad, S.
dc.creatorHafiz Muhammad Suleman
dc.creatorMahmood, Z.
dc.creatorAli, M.
dc.creatorAziz, A.
dc.creatorRasheed, A.
dc.creatorHuihui Li
dc.date2023-01-25T01:20:24Z
dc.date2023-01-25T01:20:24Z
dc.date2022
dc.date.accessioned2023-07-17T20:10:16Z
dc.date.available2023-07-17T20:10:16Z
dc.identifierhttps://hdl.handle.net/10883/22464
dc.identifier10.3389/fpls.2022.972481
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7514209
dc.descriptionIncreasing global temperature has adverse effects on crop health and productivity at both seedling and reproductivity stages. It is paramount to develop heat tolerant wheat cultivars able to sustain under high and fluctuating temperature conditions. An experiment was conducted to characterize 194 historical wheat cultivars of Pakistan under high temperature at seedling stage to identify loci associated with heat tolerance using genome-wide association studies (GWAS). A quantitative trait locus, TaHST1, on chr4A was also characterized to identify the haplotypes at this locus associated with heat tolerance in wheat from Pakistan. Initially, the diversity panel was planted under control conditions (25°C/20°C day and night temperature) in a glass house. At three leaf stage, plants were subjected to heat stress (HS) by increasing temperature (40°C/35°C day and night), while one treatment was kept at control condition. After 7 days of HS, data were collected for seedling morphology. Heat stress reduced these traits by 25% (root weight) to 40% (shoot weight), and shoot biomass was largely affected by heat stress. A GWAS model, fixed and random model circulating probability unification (FarmCPU), identified 43 quantitative trait nucleotides (QTNs) on all chromosomes, except chr7B, were associated under both HS and control conditions. Thirteen QTNs were identified in control, while 30 QTNs were identified in HS condition. In total, 24 haplotypes were identified at TaHST1 locus, and most of the heat tolerant genotypes were assigned to Hap-20 and Hap-21. Eleven QTNs were identified within 0.3–3.1 Mb proximity of heat shock protein (HSP). Conclusively, this study provided a detailed genetic framework of heat tolerance in wheat at the seedling stage and identify potential genetic regions associated with heat tolerance which can be used for marker assisted selection (MAS) in breeding for heat stress tolerance.
dc.languageEnglish
dc.publisherFrontiers
dc.relationhttps://figshare.com/collections/Genome-wide_association_for_heat_tolerance_at_seedling_stage_in_historical_spring_wheat_cultivars/6165252
dc.relationClimate adaptation & mitigation
dc.relationNutrition, health & food security
dc.relationAccelerated Breeding
dc.relationGenetic Innovation
dc.relationNational Science Foundation of China
dc.relationProject of Hainan Yazhou Bay Seed Laboratory
dc.relationCGIAR Trust Fund
dc.relationhttps://hdl.handle.net/10568/128239
dc.rightsCIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose
dc.rightsOpen Access
dc.source13
dc.source1664-462X
dc.sourceFrontiers in Plant Science
dc.source972481
dc.subjectAGRICULTURAL SCIENCES AND BIOTECHNOLOGY
dc.subjectHeat Stress Tolerance
dc.subjectQuantitative Trait Nucleotides
dc.subjectSingle Nucleotide Polymorphism Markers
dc.subjectHEAT STRESS
dc.subjectQUANTITATIVE TRAIT LOCI
dc.subjectNUCLEOTIDES
dc.subjectSINGLE NUCLEOTIDE POLYMORPHISM
dc.subjectSPRING WHEAT
dc.subjectWheat
dc.titleGenome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars
dc.typeArticle
dc.typePublished Version
dc.coverageSwitzerland


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