QTL mapping of seedling and field resistance to stem rust in DAKIYE/Reichenbachii durum wheat population

dc.creatorMegerssa, S.H.
dc.creatorAmmar, K.
dc.creatorAcevedo, M.
dc.creatorBergstrom, G.C.
dc.creatorDreisigacker, S.
dc.creatorRandhawa, M.S.
dc.creatorBrown-Guedira, G.
dc.creatorWard, B.
dc.creatorSorrells, M.E.
dc.date2022-12-07T20:30:17Z
dc.date2022-12-07T20:30:17Z
dc.date2022
dc.date.accessioned2023-07-17T20:09:46Z
dc.date.available2023-07-17T20:09:46Z
dc.identifierhttps://hdl.handle.net/10883/22296
dc.identifier10.1371/journal.pone.0273993
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7514047
dc.descriptionStem rust caused by the fungus Puccinia graminis f.sp. tritici Eriks. & E. Henn. (Pgt) threatens the global production of both durum wheat (Triticum turgidum L. ssp. durum (Desf.) Husnot) and common wheat (Triticum aestivum L.). The objective of this study was to evaluate a durum wheat recombinant inbred line (RIL) population from a cross between a susceptible parent ‘DAKIYE’ and a resistant parent ‘Reichenbachii’ developed by the International Center for the Improvement of Maize and Wheat (CIMMYT) 1) for seedling response to races JRCQC and TTRTF and 2) for field response to a bulk of the current Pgt races prevalent in Ethiopia and Kenya and 3) to map loci associated with seedling and field resistances in this population. A total of 224 RILs along with their parents were evaluated at the seedling stage in the Ethiopian Institute for Agricultural Research greenhouse at Debre Zeit, Ethiopia and in the EIAR and KALRO fields in Ethiopia and Kenya, for two seasons from 2019 to 2020. The lines were genotyped using the genotyping-by-sequencing approach. A total of 843 single nucleotide polymorphism markers for 175 lines were used for quantitative trait locus (QTL) analyses. Composite interval mapping (CIM) identified three QTL on chromosomes 3B, 4B and 7B contributed by the resistant parent. The QTL on chromosome 3B was identified at all growth stages and it explained 11.8%, 6.5%, 6.4% and 15.3% of the phenotypic variation for responses to races JRCQC, TTRTF and in the field trials ETMS19 and KNMS19, respectively. The power to identify additional QTL in this population was limited by the number of high-quality markers, since several markers with segregation distortion were eliminated. A cytological study is needed to understand the presence of chromosomal rearrangements. Future evaluations of additional durum lines and RIL families identification of durable adult plant resistance sources is crucial for breeding stem rust resistance in durum wheat in the future.
dc.languageEnglish
dc.publisherPublic Library of Science
dc.relationhttps://figshare.com/articles/figure/Distribution_of_alleles_from_the_susceptible_parent_DAKIYE_coded_as_A_and_the_resistant_parent_Reichenbachii_coded_as_B_/21289106
dc.relationNutrition, health & food security
dc.relationAccelerated Breeding
dc.relationGenetic Innovation
dc.relationUK Aid from the British People
dc.relationBill & Melinda Gates Foundation
dc.relationhttps://hdl.handle.net/10568/126707
dc.rightsCIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose
dc.rightsOpen Access
dc.source10
dc.source17
dc.source1932-6203
dc.sourcePLoS ONE
dc.sourcee0273993
dc.subjectAGRICULTURAL SCIENCES AND BIOTECHNOLOGY
dc.subjectRecombinant Inbred Lines
dc.subjectGenotyping by Sequencing
dc.subjectBREEDING
dc.subjectCHROMOSOMES
dc.subjectGENETIC MARKERS
dc.subjectGENOTYPING
dc.subjectGREENHOUSES
dc.subjectGROWTH
dc.subjectMAIZE
dc.subjectPHENOTYPIC VARIATION
dc.subjectPLANT RESPONSE
dc.subjectQUANTITATIVE ANALYSIS
dc.subjectQUANTITATIVE TRAIT LOCI MAPPING
dc.subjectSEEDLINGS
dc.subjectSINGLE NUCLEOTIDE POLYMORPHISM
dc.subjectSTEM RUST
dc.subjectWHEAT
dc.subjectBASIDIOMYCOTA
dc.subjectDISEASE RESISTANCE
dc.subjectGENETICS
dc.subjectMICROBIOLOGY
dc.subjectPLANT BREEDING
dc.titleQTL mapping of seedling and field resistance to stem rust in DAKIYE/Reichenbachii durum wheat population
dc.typeArticle
dc.typePublished Version
dc.coverageSan Francisco, CA (USA)


Este ítem pertenece a la siguiente institución