Resistance response of drought and heat tolerant spring wheat lines against the cereal cyst nematode, heterodera filipjevi

dc.creatorAtiya, O.
dc.creatorDababat, A.A.
dc.creatorErginbas Orakci, G.
dc.creatorSukhwinder-Singh
dc.creatorToktay, H.
dc.date2022-11-11T01:20:14Z
dc.date2022-11-11T01:20:14Z
dc.date2022
dc.date.accessioned2023-07-17T20:09:41Z
dc.date.available2023-07-17T20:09:41Z
dc.identifierhttps://hdl.handle.net/10883/22255
dc.identifier10.15832/ankutbd.818047
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7514010
dc.descriptionSpot blotch caused by Bipolaris sorokiniana is a destructive disease of wheat worldwide. This study investigated the aggressiveness of B. sorokiniana isolates from different wheat-growing areas of Bolu province in Turkey on the cultivar Seri-82. Host susceptibility of 55 wheat cultivars was evaluated against the most aggressive isolate. Our results indicated that the cultivars Anafarta and Koç-2015 were the most resistant. A specific and sensitive qPCR assay was developed for detecting the pathogen in plant tissues and evaluating wheat plants with different resistance levels. Three primer sets, BsGAPDHF/BsGAPDHR, BsITSF/BsITSR, and BsSSUF/BsSSUR, were designed based on glyceraldehyde-3-phosphate dehydrogenase, internal transcribed spacers, and 18S rRNA loci of B. sorokiniana with detection limits of 1, 0.1, and 0.1 pg of pathogen DNA, respectively. The qPCR assay was highly sensitive and did not amplify DNA from the other closely related fungal species and host plants. The protocol differentiated wheat plants with varying degrees of resistance. The assay developed a useful tool for the quantification of the pathogen in the early stages of infection and may provide a significant contribution to a more efficient selection of wheat genotypes in breeding studies. In the present study, expression levels of PR proteins, phenylalanine ammonia-lyase, catalase, ascorbate peroxidase, and superoxide dismutase enzymes were upregulated in Anafarta (resistant) and Nenehatun (susceptible) cultivars at different post-infection time points, but more induced in the susceptible cultivar. The results showed considerable variation in the expression levels and timing of defense genes in both cultivars.
dc.description326-341
dc.languageEnglish
dc.publisherAnkara University
dc.relationNutrition, health & food security
dc.relationAccelerated Breeding
dc.relationGenetic Innovation
dc.relationCGIAR Trust Fund
dc.relationhttps://hdl.handle.net/10568/126528
dc.rightsCIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose
dc.rightsOpen Access
dc.source2
dc.source28
dc.source1300-7580
dc.sourceJournal of Agricultural Sciences
dc.subjectAGRICULTURAL SCIENCES AND BIOTECHNOLOGY
dc.subjectPlant Parasitic Nematodes
dc.subjectResistant Source
dc.subjectBIOTIC STRESS
dc.subjectBREEDING
dc.subjectCEREAL CROPS
dc.subjectPLANT NEMATODES
dc.subjectSPRING WHEAT
dc.subjectDROUGHT RESISTANCE
dc.titleResistance response of drought and heat tolerant spring wheat lines against the cereal cyst nematode, heterodera filipjevi
dc.typeArticle
dc.typePublished Version
dc.coverageTurkey


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