Genotyping by sequencing advancements in barley

dc.creatorRajendran, N.R.
dc.creatorQureshi, N.
dc.creatorPourkheirandish, M.
dc.date2022-09-24T00:20:12Z
dc.date2022-09-24T00:20:12Z
dc.date2022
dc.date.accessioned2023-07-17T20:09:29Z
dc.date.available2023-07-17T20:09:29Z
dc.identifierhttps://hdl.handle.net/10883/22193
dc.identifier10.3389/fpls.2022.931423
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7513949
dc.descriptionBarley is considered an ideal crop to study cereal genetics due to its close relationship with wheat and diploid ancestral genome. It plays a crucial role in reducing risks to global food security posed by climate change. Genetic variations in the traits of interest in crops are vital for their improvement. DNA markers have been widely used to estimate these variations in populations. With the advancements in next-generation sequencing, breeders could access different types of genetic variations within different lines, with single-nucleotide polymorphisms (SNPs) being the most common type. However, genotyping barley with whole genome sequencing (WGS) is challenged by the higher cost and computational demand caused by the large genome size (5.5GB) and a high proportion of repetitive sequences (80%). Genotyping-by-sequencing (GBS) protocols based on restriction enzymes and target enrichment allow a cost-effective SNP discovery by reducing the genome complexity. In general, GBS has opened up new horizons for plant breeding and genetics. Though considered a reliable alternative to WGS, GBS also presents various computational difficulties, but GBS-specific pipelines are designed to overcome these challenges. Moreover, a robust design for GBS can facilitate the imputation to the WGS level of crops with high linkage disequilibrium. The complete exploitation of GBS advancements will pave the way to a better understanding of crop genetics and offer opportunities for the successful improvement of barley and its close relatives.
dc.languageEnglish
dc.publisherFrontiers Media S.A.
dc.relationNutrition, health & food security
dc.relationAccelerated Breeding
dc.relationGenetic Innovation
dc.relationCGIAR Trust Fund
dc.relationhttps://hdl.handle.net/10568/129085
dc.rightsCIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose
dc.rightsOpen Access
dc.source13
dc.source1664-462X
dc.sourceFrontiers in Plant Science
dc.source931423
dc.subjectAGRICULTURAL SCIENCES AND BIOTECHNOLOGY
dc.subjectGenotyping by Sequencing
dc.subjectTarget Enrichment
dc.subjectBARLEY
dc.subjectGENOTYPING
dc.subjectRESTRICTION ENZYMES
dc.subjectSINGLE NUCLEOTIDE POLYMORPHISM
dc.titleGenotyping by sequencing advancements in barley
dc.typeArticle
dc.typePublished Version
dc.coverageSwitzerland


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