Salivary effector Sm9723 of grain aphid sitobion miscanthi suppresses plant defense and is essential for aphid survival on wheat

dc.creatorYong Zhang
dc.creatorLiu, X.
dc.creatorYu Fu
dc.creatorCrespo Herrera, L.A.
dc.creatorHuan Liu
dc.creatorWang, Q
dc.creatorZhang, Y.
dc.creatorChen, J.
dc.date2022-07-15T00:20:14Z
dc.date2022-07-15T00:20:14Z
dc.date2022
dc.date.accessioned2023-07-17T20:09:19Z
dc.date.available2023-07-17T20:09:19Z
dc.identifierhttps://hdl.handle.net/10883/22109
dc.identifier10.3390/ijms23136909
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7513872
dc.descriptionAphid salivary effectors play important roles in modulating plant defense responses. The grain aphid Sitobion miscanthi is one of the most economically important cereal aphids worldwide. However, little information is available on the identification and functional analysis of salivary effectors of S. miscanthi. In this study, a candidate salivary effector Sm9723 was identified, which was specifically expressed in aphid salivary glands and highly induced during the aphid feeding phase. Transient overexpression of Sm9723 in Nicotiana benthamiana suppressed BAX and INF1-induced cell death. Further, Sm9723 overexpression inhibited N. benthamiana defense responses by reducing pattern-triggered immunity associated callose deposition and expression levels of jasmonic and salicylic acid-associated defense genes. In addition, the salivary effector Sm9723 of S. miscanthi was effectively silenced through nanocarrier-mediated dsRNA delivery system. After silencing Sm9723, fecundity and survival of S. miscanthi decreased significantly, and the aphid feeding behavior was also negatively affected. These results suggest salivary effector Sm9723 is involved in suppressing plant immunity and is essential in enabling aphid virulence, which could be applied as potential target gene for RNAi-mediated pest control of S. miscanthi.
dc.languageEnglish
dc.publisherMDPI
dc.relationhttps://www.mdpi.com/article/10.3390/ijms23136909/s1
dc.relationNutrition, health & food security
dc.relationAccelerated Breeding
dc.relationGenetic Innovation
dc.relationNational Natural Science Foundation of China
dc.relationScientific Cooperation Project of MARA-CABI Joint Laboratory for Bio-safety
dc.relationhttps://hdl.handle.net/10568/129170
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dc.rightsOpen Access
dc.source13
dc.source23
dc.source1661-6596
dc.sourceInternational Journal of Molecular Sciences
dc.source6909
dc.subjectAGRICULTURAL SCIENCES AND BIOTECHNOLOGY
dc.subjectCereal Aphid
dc.subjectSalivary Protein
dc.subjectPlant Immunity
dc.subjectRNA Interference
dc.subjectAphid Performance
dc.subjectAPHIDOIDEA
dc.subjectPLANTS
dc.subjectRNA
dc.subjectPROTEINS
dc.titleSalivary effector Sm9723 of grain aphid sitobion miscanthi suppresses plant defense and is essential for aphid survival on wheat
dc.typeArticle
dc.typePublished Version
dc.coverageBasel (Switzerland)


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