Pearl Millet Aquaporin Gene PgPIP2;6 Improves Abiotic Stress Tolerance in Transgenic Tobacco

dc.creatorPalakolanu, S.R.
dc.creatorDhaware, M.G,
dc.creatorSivasakthi, K.
dc.creatorDivya, K.
dc.creatorNagaraju, M.
dc.creatorKatamreddy Sri Cindhuri
dc.creatorKavi Kishor Bilhan Polavarapu
dc.creatorBhatnagar-Mathur, P.
dc.creatorVadez, V.
dc.creatorSharma, K.K.
dc.date2022-04-27T00:00:17Z
dc.date2022-04-27T00:00:17Z
dc.date2022
dc.date.accessioned2023-07-17T20:09:08Z
dc.date.available2023-07-17T20:09:08Z
dc.identifierhttps://hdl.handle.net/10883/22051
dc.identifier10.3389/fpls.2022.820996
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7513816
dc.descriptionPearl millet [Pennisetum glaucum (L) R. Br.] is an important cereal crop of the semiarid tropics, which can withstand prolonged drought and heat stress. Considering an active involvement of the aquaporin (AQP) genes in water transport and desiccation tolerance besides several basic functions, their potential role in abiotic stress tolerance was systematically characterized and functionally validated. A total of 34 AQP genes from P. glaucum were identified and categorized into four subfamilies, viz., plasma membrane intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs), nodulin-26-like intrinsic proteins (NIPs), and small basic intrinsic proteins (SIPs). Sequence analysis revealed that PgAQPs have conserved characters of AQP genes with a closer relationship to sorghum. The PgAQPs were expressed differentially under high vapor pressure deficit (VPD) and progressive drought stresses where the PgPIP2;6 gene showed significant expression under high VPD and drought stress. Transgenic tobacco plants were developed by heterologous expression of the PgPIP2;6 gene and functionally characterized under different abiotic stresses to further unravel their role. Transgenic tobacco plants in the T2 generations displayed restricted transpiration and low root exudation rates in low- and high-VPD conditions. Under progressive drought stress, wild-type (WT) plants showed a quick or faster decline of soil moisture than transgenics. While under heat stress, PgPIP2;6 transgenics showed better adaptation to heat (40°C) with high canopy temperature depression (CTD) and low transpiration; under low-temperature stress, they displayed lower transpiration than their non-transgenic counterparts. Cumulatively, lower transpiration rate (Tr), low root exudation rate, declined transpiration, elevated CTD, and lower transpiration indicate that PgPIP2;6 plays a role under abiotic stress tolerance. Since the PgPIP2;6 transgenic plants exhibited better adaptation against major abiotic stresses such as drought, high VPD, heat, and cold stresses by virtue of enhanced transpiration efficiency, it has the potential to engineer abiotic stress tolerance for sustained growth and productivity of crops.
dc.languageEnglish
dc.publisherFrontiers
dc.rightsCIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose
dc.rightsOpen Access
dc.source13
dc.source1664-462X
dc.sourceFrontiers in Plant Science
dc.source820996
dc.subjectAGRICULTURAL SCIENCES AND BIOTECHNOLOGY
dc.subjectCanopy Temperature Depression
dc.subjectExudation Rate
dc.subjectPgPIP2;6
dc.subjectProgressive Drought Stress
dc.subjectTranspiration Rate
dc.subjectTranspiration Efficiency
dc.subjectVapor Pressure Deficit
dc.subjectEXUDATION
dc.subjectPENNISETUM GLAUCUM
dc.subjectTRANSPIRATION
dc.subjectNICOTIANA TABACUM
dc.subjectPROTEINS
dc.titlePearl Millet Aquaporin Gene PgPIP2;6 Improves Abiotic Stress Tolerance in Transgenic Tobacco
dc.typeArticle
dc.typePublished Version
dc.coverageSwitzerland


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