Genome-Wide Association Study of Phytic Acid in Wheat Grain Unravels Markers for Improving Biofortification

dc.creatorZhengyu Wen
dc.creatorJuliana, P.
dc.creatorDhugga, H. S.
dc.creatorPacheco, M.
dc.creatorMartínez, U.I.
dc.creatorAguilar, A.
dc.creatorIbba, M.I.
dc.creatorVelu, G.
dc.creatorSingh, R.P.
dc.creatorDhugga, K.
dc.date2022-02-05T01:20:15Z
dc.date2022-02-05T01:20:15Z
dc.date2022
dc.date.accessioned2023-07-17T20:08:53Z
dc.date.available2023-07-17T20:08:53Z
dc.identifierhttps://hdl.handle.net/10883/21954
dc.identifier10.3389/fpls.2022.830147
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7513721
dc.descriptionBiofortification of cereal grains offers a lasting solution to combat micronutrient deficiency in developing countries where it poses developmental risks to children. Breeding efforts thus far have been directed toward increasing the grain concentrations of iron (Fe) and zinc (Zn) ions. Phytic acid (PA) chelates these metal ions, reducing their bioavailability in the digestive tract. We present a high-throughput assay for quantification of PA and its application in screening a breeding population. After extraction in 96-well megatiter plates, PA content was determined from the phosphate released after treatment with a commercially available phytase enzyme. In a set of 330 breeding lines of wheat grown in the field over 3 years as part of a Harvest Plus breeding program for high grain Fe and Zn, our assay unraveled variation for PA that ranged from 0.90 to 1.72% with a mean of 1.24%. PA content was not associated with grain yield. High yielding lines were further screened for low molar PA/Fe and PA/Zn ratios for increased metal ion bioavailability, demonstrating the utility of our assay. Genome-wide association study revealed 21 genetic associations, six of which were consistent across years. Five of these associations mapped to chromosomes 1A, 2A, 2D, 5A, and 7D. Additivity over four of these haplotypes accounted for an ∼10% reduction in PA. Our study demonstrates it is possible to scale up assays to directly select for low grain PA in forward breeding programs.
dc.languageEnglish
dc.publisherFrontiers
dc.relationhttps://figshare.com/collections/Genome-Wide_Association_Study_of_Phytic_Acid_in_Wheat_Grain_Unravels_Markers_for_Improving_Biofortification/5842550
dc.relationNutrition, health & food security
dc.relationAccelerated Breeding
dc.relationGenetic Innovation
dc.relationCGIAR Research Program on Wheat
dc.relationHarvest Plus
dc.relationBill & Melinda Gates Foundation
dc.relationForeign, Commonwealth & Development Office
dc.relationhttps://hdl.handle.net/10568/126372
dc.rightsCIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose
dc.rightsOpen Access
dc.source13
dc.source1664-462X
dc.sourceFrontiers in Plant Science
dc.source830147
dc.subjectAGRICULTURAL SCIENCES AND BIOTECHNOLOGY
dc.subjectBIOFORTIFICATION
dc.subjectGRAIN
dc.subjectNUTRITION
dc.subjectIRON
dc.subjectZINC
dc.subjectPHYTIC ACID
dc.subjectGENOMES
dc.titleGenome-Wide Association Study of Phytic Acid in Wheat Grain Unravels Markers for Improving Biofortification
dc.typeArticle
dc.typePublished Version
dc.coverageSwitzerland


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