dc.creatorIge, A.D.
dc.creatorOlasanmi, B.
dc.creatorMbanjo, E.G.N.
dc.creatorKayondo, I.S.
dc.creatorParkes, E.
dc.creatorKulakow, P.
dc.creatorEgesi, C.N.
dc.creatorBauchet, G.J.
dc.creatorEng Hwa Ng
dc.creatorBecerra Lopez-Lavalle, L.A.
dc.creatorCeballos, H.
dc.creatorRabbi, I.
dc.date2021-07-10T00:10:15Z
dc.date2021-07-10T00:10:15Z
dc.date2021
dc.date.accessioned2023-07-17T20:07:50Z
dc.date.available2023-07-17T20:07:50Z
dc.identifierhttps://hdl.handle.net/10883/21566
dc.identifier10.3390/agronomy11030420
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7513347
dc.descriptionCassava mosaic disease (CMD) is a major viral disease adversely affecting cassava production in Africa and Asia. Genomic regions conferring resistance to the disease have been mapped in African cassava germplasm through biparental quantitative trait loci (QTL) mapping and genome-wide association studies. To facilitate the utilization of these markers in breeding pipelines to support selections, proof-of-concept technical and biological validation research was carried out using independent pre-breeding and breeding populations. Kompetitive Allele-Specific Polymerase Chain Reaction (KASP) assays were designed from three single nucleotide polymorphism (SNP) markers linked to a major resistance locus on chromosome 12 (S12_7926132, S12_7926163) and a minor locus on chromosome 14 (S14_4626854). The designed assays were robust and easy to score with >99% genotype call rate. The overall predictive accuracy (proportion of true positives and true negatives) of the markers (S12_7926132 and S14_4626854) was 0.80 and 0.78 in the pre-breeding and breeding population, respectively. On average, genotypes that carried at least one copy of the resistant allele at the major CMD2 locus had a significantly higher yield advantage. Nevertheless, variation was observed in prediction accuracies for the major locus (S12_7926132) among sub-families from the two populations, suggesting the need for context-specific utilization, for example, by screening for co-segregation of favorable SNP alleles with resistance in the parents being used for crosses. Availability of these validated SNP markers on the uniplex KASP genotyping platform represents an important step in translational genetics toward marker-assisted selection to accelerate introgression of favorable resistant alleles in breeding populations.
dc.languageEnglish
dc.publisherMDPI
dc.relationhttps://www.mdpi.com/2073-4395/11/3/420#supplementary
dc.rightsCIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose
dc.rightsOpen Access
dc.source3
dc.source11
dc.source2073-4395
dc.sourceAgronomy
dc.source420
dc.subjectAGRICULTURAL SCIENCES AND BIOTECHNOLOGY
dc.subjectCMD Resistance
dc.subjectMarker Predictive Accuracy
dc.subjectAllele Specific PCR Assay
dc.subjectCASSAVA
dc.subjectDISEASE RESISTANCE
dc.subjectMARKER-ASSISTED SELECTION
dc.subjectPCR
dc.subjectALLELES
dc.titleConversion and validation of uniplex SNP markers for selection of resistance to cassava mosaic disease in cassava breeding programs
dc.typeArticle
dc.typePublished Version
dc.coverageBasel (Switzerland)


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