dc.creatorTonui, R.
dc.creatorMasanga, J.
dc.creatorKasili, R.
dc.creatorRuno, S.
dc.creatorAlakonya, A.
dc.date2020-12-19T01:10:14Z
dc.date2020-12-19T01:10:14Z
dc.date2020
dc.date.accessioned2023-07-17T20:06:30Z
dc.date.available2023-07-17T20:06:30Z
dc.identifierhttps://hdl.handle.net/10883/21080
dc.identifier10.1111/jph.12908
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7512866
dc.descriptionMaize lethal necrosis disease (MLND) is a devastating viral disease of maize caused by double infection with Maize chlorotic mottle virus (MCMV) and any one of the Potyviridae family members. Management of MLND requires effective resistance screening and surveillance tools. In this study, we report the use of small RNA (sRNA) profiling to detect MLND causal viruses and further the development of alternative detection markers for use in routine surveillance of the disease-causing viruses. Small RNAs (sRNAs) originating from five viruses namely MCMV, Sugarcane mosaic virus (SCMV), Maize streak virus (MSV), Maize-associated totivirus (MATV) and Maize yellow mosaic virus (MYMV) were assembled from infected maize samples collected from MLND hot spots in Kenya. The expression of the identified viral domains was further validated using quantitative real-time PCR. New markers for the detection of some of the MLND causal viruses were also developed from the highly expressed domains and used to detect the MLND-causative viruses in maize and alternative hosts. These findings further demonstrate the potential of using sRNAs especially from highly expressed viral motifs in the detection of MLND causal viruses. We report the validation of new sets of primers for use in detection of the most common MLND causal viruses MCMV and SCMV in East Africa.
dc.description439-450
dc.languageEnglish
dc.publisherWiley
dc.relationhttps://onlinelibrary.wiley.com/doi/full/10.1111/jph.12908#support-information-section
dc.rightsCIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose
dc.rightsOpen Access
dc.source7-8
dc.source168
dc.source1439-0434
dc.sourceJournal of Phytopathology
dc.subjectAGRICULTURAL SCIENCES AND BIOTECHNOLOGY
dc.subjectMaize Lethal Necrosis
dc.subjectMicroRNA
dc.subjectSmall Interfering RNA
dc.subjectSmall RNA Sequencing
dc.subjectMAIZE CHLOROTIC MOTTLE VIRUS
dc.subjectRNA
dc.subjectRNA SEQUENCE
dc.subjectSUGARCANE MOSAIC VIRUS
dc.titleIdentification of maize lethal necrosis disease causal viruses in maize and suspected alternative hosts through small RNA profiling
dc.typeArticle
dc.typePublished Version
dc.coverageUnited Kingdom


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