Rapid identification and characterization of genetic loci for defective kernel in bread wheat

dc.creatorChao Fu
dc.creatorJiuyuan Du
dc.creatorXiuling Tian
dc.creatorHe Zhonghu
dc.creatorLuping Fu
dc.creatorYue Wang
dc.creatorDengan Xu
dc.creatorXiaoting Xu
dc.creatorXianchun Xia
dc.creatorZhang Yan
dc.creatorShuanghe Cao
dc.date2019-12-04T01:20:18Z
dc.date2019-12-04T01:20:18Z
dc.date2019
dc.date.accessioned2023-07-17T20:05:02Z
dc.date.available2023-07-17T20:05:02Z
dc.identifier1471-2229 (Print)
dc.identifierhttps://hdl.handle.net/10883/20472
dc.identifier10.1186/s12870-019-2102-6
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7512284
dc.descriptionBackground: Wheat is a momentous crop and feeds billions of people in the world. The improvement of wheat yield is very important to ensure world food security. Normal development of grain is the essential guarantee for wheat yield formation. The genetic study of grain phenotype and identification of key genes for grain filling are of great significance upon dissecting the molecular mechanism of wheat grain morphogenesis and yield potential. Results: Here we identified a pair of defective kernel (Dek) isogenic lines, BL31 and BL33, with plump and shrunken mature grains, respectively, and constructed a genetic population from the BL31/BL33 cross. Ten chromosomes had higher frequency of polymorphic single nucleotide polymorphism (SNP) markers between BL31 and BL33 using Wheat660K chip. Totally 783 simple sequence repeat (SSR) markers were chosen from the above chromosomes and 15 of these were integrated into two linkage groups using the genetic population. Genetic mapping identified three QTL, QDek.caas-3BS.1, QDek.caas-3BS.2 and QDek.caas-4AL, explaining 14.78-18.17%, 16.61-21.83% and 19.08-28.19% of phenotypic variances, respectively. Additionally, five polymorphic SNPs from Wheat660K were successfully converted into cleaved amplified polymorphic sequence (CAPS) markers and enriched the target regions of the above QTL. Biochemical analyses revealed that BL33 has significantly higher grain sucrose contents at filling stages and lower mature grain starch contents than BL31, indicating that the Dek QTL may be involved in carbohydrate metabolism. As such, the candidate genes for each QTL were predicated according to International Wheat Genome Sequence Consortium (IWGSC) RefSeq v1.0. Conclusions: Three major QTL for Dek were identified and their causal genes were predicted, laying a foundation to conduct fine mapping and dissect the regulatory mechanism underlying Dek trait in wheat.
dc.formatPDF
dc.languageEnglish
dc.publisherBioMed Central
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dc.rightsOpen Access
dc.source1
dc.sourceart. 483
dc.source19
dc.sourceBMC Plant Biology
dc.subjectKERNELS
dc.subjectSEED FILLING
dc.subjectQUANTITATIVE TRAIT LOCI
dc.subjectSINGLE NUCLEOTIDE POLYMORPHISM
dc.subjectTRITICUM AESTIVUM
dc.titleRapid identification and characterization of genetic loci for defective kernel in bread wheat
dc.typeArticle
dc.typePublished Version
dc.coverageLondon (United Kingdom)


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