Novel molecular marker-assisted strategy for production of wheat-Leymus mollis chromosome addition lines

dc.creatorEdet, O.U.
dc.creatorGorafi, Y.S.A
dc.creatorSeong-woo Cho
dc.creatorKishii, M.
dc.creatorHisashi Tsujimoto
dc.date2019-01-17T23:32:35Z
dc.date2019-01-17T23:32:35Z
dc.date2018
dc.date.accessioned2023-07-17T20:03:31Z
dc.date.available2023-07-17T20:03:31Z
dc.identifierESSN: 2045-2322
dc.identifierhttps://hdl.handle.net/10883/19824
dc.identifier10.1038/s41598-018-34545-x
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7511688
dc.descriptionDeveloping wheat–alien chromosome introgression lines to improve bread wheat’s resistance to stresses, such as drought, salinity stress and diseases, requires reliable markers to identify and characterize the alien chromatins. Leymus mollis is a wild relative of bread wheat resistant to salinity and economically important diseases of wheat, but its genome sequence and cytological markers are not available. We devised a molecular marker-assisted strategy for L. mollis chromosome identification and applied it to produce 10 wheat–L. mollis chromosome addition lines. Using 47L. racemosus genome polymorphic PCR markers and DArTseq genotyping, we distinguished the L. mollis chromosomes and differentiated disomic and monosomic lines by progeny test. DArTseq genotyping generated 14,530L. mollis SNP markers and the chromosome-specifc SNP markers were used to determine the homoeologous groups of L. mollis chromosomes in the addition lines. To validate the marker-based results, genomic in situ hybridization was applied to confirm the presence and cytological status of L. mollis chromosomes in the lines. This study demonstrates that adequate molecular markers allow the production and characterization of wheat–alien addition lines without in situ hybridization, which saves considerable time and effort.
dc.formatPDF
dc.languageEnglish
dc.publisherSpringer Nature
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dc.rightsOpen Access
dc.sourceart. 16117
dc.source8
dc.sourceScientific Reports
dc.subjectAGRICULTURAL SCIENCES AND BIOTECHNOLOGY
dc.subjectGenetic Hybridization
dc.subjectGENETIC MARKERS
dc.subjectPLANT BREEDING
dc.titleNovel molecular marker-assisted strategy for production of wheat-Leymus mollis chromosome addition lines
dc.typeArticle
dc.coverageUnited Kingdom


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