Validation of consensus quantitative trait loci associated with resistance to multiple foliar pathogens of maize

dc.creatorAsea, G.
dc.creatorVivek, B.
dc.creatorBigirwa, G.
dc.creatorLipps, P.E.
dc.creatorPratt, R.C.
dc.date2013-06-30T05:24:34Z
dc.date2013-06-30T05:24:34Z
dc.date2009
dc.date.accessioned2023-07-17T19:57:09Z
dc.date.available2023-07-17T19:57:09Z
dc.identifier0031-949X
dc.identifier1943-7684
dc.identifierhttp://hdl.handle.net/10883/3081
dc.identifier10.1094/PHYTO-99-5-0540
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7509032
dc.descriptionMaize production in sub-Saharan Africa incurs serious losses to epiphytotics of foliar diseases. Quantitative trait loci conditioning partial resistance (rQTL) to infection by causal agents of gray leaf spot (GLS), northern corn leaf blight (NCLB), and maize streak have been reported. Our objectives were to identify simple-sequence repeat (SSR) molecular markers linked to consensus rQTL and one recently identified rQTL associated with GLS, and to determine their suitability as tools for selection of improved host resistance. We conducted evaluations of disease severity phenotypes in separate field nurseries, each containing 410 F2:3 families derived from a cross between maize inbred CML202 (NCLB and maize streak resistant) and VP31 (a GLS-resistant breeding line) that possess complimentary rQTL. F2:3 families were selected for resistance based on genotypic (SSR marker), phenotypic, or combined data and the selected F3:4 families were reevaluated. Phenotypic values associated with SSR markers for consensus rQTL in bins 4.08 for GLS, 5.04 for NCLB, and 1.04 for maize streak significantly reduced disease severity in both generations based on single-factor analysis of variance and marker-interval analysis. These results were consistent with the presence of homozygous resistant parent alleles, except in bin 8.06, where markers were contributed by the NCLB-susceptible parent. Only one marker associated with resistance could be confirmed in bins 2.09 (GLS) and 3.06 (NCLB), illustrating the need for more robust rQTL discovery, fine-mapping, and validation prior to undertaking marker-based selection.
dc.description540-547
dc.formatPDF
dc.languageEnglish
dc.publisherAmerican Phytopathological Society (APS)
dc.rightsCIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.
dc.rightsOpen Access
dc.source5
dc.source99
dc.sourcePhytopathology
dc.subjectAGRICULTURAL SCIENCES AND BIOTECHNOLOGY
dc.subjectMAIZE
dc.subjectQUANTITATIVE TRAIT LOCI
dc.subjectDISEASE RESISTANCE
dc.titleValidation of consensus quantitative trait loci associated with resistance to multiple foliar pathogens of maize
dc.typeArticle


Este ítem pertenece a la siguiente institución