An Enzymatic–Colorimetric Assay for the Quantification of <i>Bifidobacterium</i>

dc.creatorBibiloni, Rodrigo
dc.creatorPérez, Pablo Fernando
dc.creatorDe Antoni, Graciela Liliana
dc.date2000-03
dc.date2022-09-12T16:49:30Z
dc.date.accessioned2023-07-15T05:13:35Z
dc.date.available2023-07-15T05:13:35Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/141899
dc.identifierissn:0362-028X
dc.identifierissn:1944-9097
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7472559
dc.descriptionAn enzymatic-colorimetric assay for the quantification of <i>Bifidobacterium</i> was developed. The method, based upon the standard detection of fructose-6-phosphate phosphoketolase activity, was optimized with respect to bacterial cell pretreatment, time of incubation, and substrate concentration. The relationship between bacterial biomass and phosphoketolase activity was linear in a wide spectrum of bacterial densities. Higher sensitivity over the standard method was achieved by using 0.25% Triton X-100 in the reaction mixture to pretreat the bacterial cells. Because autoaggregation is a frequent feature among <i>Bifidobacterium</i> strains, this simple and reproducible method offers good advantage over viable plate count and turbidimetric techniques. The methodology can also be applied to the assessment of adherent <i>Bifidobacterium</i> strains to human epithelial cells.
dc.descriptionCentro de Investigación y Desarrollo en Criotecnología de Alimentos
dc.formatapplication/pdf
dc.format322-326
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.subjectCiencias Exactas
dc.subjectQuímica
dc.subjectBifidobacterium
dc.subjectenzymatic-colorimetric assay
dc.subjectBacteria
dc.titleAn Enzymatic–Colorimetric Assay for the Quantification of <i>Bifidobacterium</i>
dc.typeArticulo
dc.typeArticulo


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