| dc.creator | Obregón, Walter David | |
| dc.creator | Arribére, María Cecilia | |
| dc.creator | Morcelle del Valle, Susana Raquel | |
| dc.creator | Liggieri, Constanza Silvina | |
| dc.creator | Caffini, Néstor Oscar | |
| dc.creator | Priolo de Lufrano, Nora Silvia | |
| dc.date | 2001 | |
| dc.date | 2022-10-11T16:59:37Z | |
| dc.date.accessioned | 2023-07-15T05:12:31Z | |
| dc.date.available | 2023-07-15T05:12:31Z | |
| dc.identifier | http://sedici.unlp.edu.ar/handle/10915/143564 | |
| dc.identifier | issn:0277-8033 | |
| dc.identifier | issn:1573-4943 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/7472491 | |
| dc.description | Two new endopeptidases were purified to homogeneity from the latex of Araujia hortorum fruits by a simple purification procedure involving ultracentrifugation and ion exchange chromatography. Molecular weights of araujiain h II and araujiain h III were 23,718 and 23546 (mass spectrometry), respectively. The isoelectric point of araujiain h II was 8.9, whereas araujiain h III had a pI higher than 9.3. Maximum proteolytic activity on caseine was reached at pH 8.0-9.0 for both endopeptidases, which were irreversibly inhibited by iodoacetate and E-64, suggesting they belong to the cysteine protease family. Esterolytic activity was determined on N-alpha-CBZ-amino acid-p-nitrophenyl esters, and the highest kcat/Km values for the both enzymes were obtained with the glutamine derivative. The N-terminal sequences of araujiain h II and araujiain h III showed a high degree of homology with other plant cysteine endopeptidases. | |
| dc.description | Centro de Investigación de Proteínas Vegetales | |
| dc.format | application/pdf | |
| dc.format | 317-325 | |
| dc.language | en | |
| dc.rights | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
| dc.rights | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
| dc.subject | Química | |
| dc.subject | Araujiain | |
| dc.subject | Cysteine endopeptidases | |
| dc.subject | Latex | |
| dc.subject | Milkweed family | |
| dc.subject | Protein purification | |
| dc.title | Two new cysteine endopeptidases obtained from the latex of Araujia hortorum fruits | |
| dc.type | Articulo | |
| dc.type | Articulo | |
