dc.creatorD'Francisco, Florencia Alina
dc.creatorLapuente, Camila
dc.creatorLópez Merlo, Mariana Lucía
dc.creatorBarbeito, Claudio Gustavo
dc.creatorGobello, María Cristina
dc.date2020
dc.date2022-02-25T15:14:29Z
dc.date.accessioned2023-07-15T05:12:10Z
dc.date.available2023-07-15T05:12:10Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/131706
dc.identifierissn:1879-3231
dc.identifierissn:0093-691X
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7472469
dc.descriptionThe aims of this study were: to ultrasonograhically describe and compare testicular parenchyma echogenicity and heterogeneity using digital image analysis in: I) prepubertal (PREP), peripubertal (PERI) and mature (MAT) cats; II) Normal and abnormal mature felids. Secondary, the relationships between histomorphological and ultrasonographic attributes of the testes were also determined. I) Fourteen, PREP, PERI and MAT male cats were ultrasonographically examined and then castrated. II) Seven adult cats were ultrasonographically examined before and after a GnRH antagonist administration and then castrated. All the testes were grossly and histomorphometrically assessed. In the frozen digital images of the longitudinal ultrasound sections, 3 regions of interest (ROI, 1 mm2) were selected. Within each ROI the echogenicity and the heterogeneity of the testicular parenchyma were digitally analyzed. In experiment I, testicular volume (0.15 ± 0.0 vs. 0.49 ± 0.1 vs. 1.65 ± 0.1; P < 0.01) and gonadosomatic index (0.04 ± 0.0 vs. 0.05 ± 0.0 vs. 0.08 ± 0.0; P < 0.01), echogenicity (56.54 ± 0.75 vs. 81.87 ± 5.88 vs.94.67 ± 3.62; P < 0.01) and heterogeneity (10.2420 ± 1.3740 vs.13.65 ± 0.65 vs. 14.67 ± 1.49; P < 0.01) augmented throughout PRE, PERI, and MAT. In experiment II, testicular volume (1.00 ± 0.09 vs. 0.85 ± 0.09; P < 0.05), echogenicity (87.74 ± 1.53 vs. 83.32 ± 1.54; P 0.01) but not heterogeneity (14.09 ± 0.26 vs. 14.19 ± 0.29; P > 0.05) decreased in the post GnRH antagonist abnormal testes. For both experiments, testicular volume, seminiferous tubular diameter, percentage of spermatids as the most mature cell type, and luminal/intertubular ratio were highly correlated (P < 0.01) with their echotextural attributes. Computer-assisted image analysis of B mode ultrasonogram appears as a good indicator of pubertal development and mild alterations of spermatogenesis in felids.
dc.descriptionFacultad de Ciencias Veterinarias
dc.formatapplication/pdf
dc.format503-507
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rightsCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.subjectCiencias Veterinarias
dc.subjectPuberty
dc.subjectAndrology
dc.subjectSubfertility
dc.subjectEcography
dc.subjectHistology
dc.subjectGerminal epithelium
dc.titleFeline testicular ultrasonogram differentiates pre vs. postpubertal and normal vs. disrupted spermatogenesis
dc.typeArticulo
dc.typePreprint


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