dc.creatorAlippi, Adriana Mónica
dc.creatorLópez, Ana Claudia
dc.creatorBalatti, Pedro Alberto
dc.date2012
dc.date2022-02-25T15:04:59Z
dc.date.accessioned2023-07-15T05:11:42Z
dc.date.available2023-07-15T05:11:42Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/131705
dc.identifierissn:0929-1873
dc.identifierissn:1573-8469
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7472439
dc.descriptionThe aim of this study was to isolate, identify and analyze the diversity of the causative agents of crown galls and hairy roots from symptomatic plants of Vaccinium corymbossum by means of biological, biochemical and molecular tools. All the bacteria isolated from blueberries (n = 78) were found to be Agrobacterium since they grew on three differential media, provoked cell and/or root proliferation on Kalanchoe, and contained a 730 bp partial sequence that codes for virulence genes within the virC operon found on Ti and/or Ri plasmids. Isolates were highly variable considering the ERIC-PCR patterns as well as biochemical reactions and were all represented by 7 different restriction patterns of the 16SrDNA. While most of the isolates belonged to Agrobacterium bv. 1 (n = 33) or Agrobacterium bv. 2 (n = 31) only fourteen were Agrobacterium rubi. A representative isolate of each of these three groups was further identified by sequencing the approximately 400 bp 16SrDNA. We concluded that Vaccinium plants are particularly susceptible to Agrobacterium bv. 1, Agrobacterium bv. 2, and also to Agrobacterium rubi. To our knowledge this is the first survey of Agrobacterium affecting blueberries in Argentina.
dc.descriptionCentro de Investigaciones en Fitopatología
dc.formatapplication/pdf
dc.format415-430
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rightsCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.subjectBotánica
dc.subjectAgrobacterium rubi
dc.subjectAgrobacterium tumefaciens
dc.subjectAgrobacterium rhizogenes
dc.subjectBiovars
dc.subjectVaccinium corymbossum
dc.subjectBlueberries
dc.subjectCrown gall disease
dc.subjectDiversity
dc.subjectPCR
dc.titleDiversity among agrobacteria isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina
dc.typeArticulo
dc.typeArticulo


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