In vitro conversion of saturated to monounsaturated fatty acid by Ehrlich ascites cells

dc.creatorMercuri, Osvaldo Francisco
dc.creatorDe Tomás, María Elena
dc.creatorAntueno, Roberto Justo de
dc.date1981
dc.date2022-11-18T13:07:45Z
dc.date.accessioned2023-07-15T05:07:05Z
dc.date.available2023-07-15T05:07:05Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/146070
dc.identifierissn:0024-4201
dc.identifierissn:1558-9307
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7472151
dc.descriptionIn this paper, evidence is presented on the capacity of Ehrlich ascites cells to synthesize in vitro monounsaturated fatty acids from radioactive palmitate. Localization of the double bond was determined by ozonolysis and subsequent reduction of the ozonides to aldesters followed by gas liquid chromatography. These results proved that Ehrlich ascites cells have a Δ9 desaturase that catalyzes the biosynthesis of palmitoleic acid from palmitic acid and oleic and vaccenic acid via elongation-desaturation and desaturation-elongation, respectively, using palmitic acid as substrate. Furthermore, it is shown that, as in the hepatic cells, Δ9 desaturase enzyme activity of the tumoral cells is associated with the endoplasmic reticulum. The electron transport components involved in the desaturase system, i.e., NADH-cytochrome bs reductase and NADH-cytochrome bs reductase, were also measured. The activities of these enzymes do not appear to be rate-limiting in the desaturase activity of these tumoral cells.
dc.descriptionFacultad de Ciencias Médicas
dc.formatapplication/pdf
dc.format893-896
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rightsCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.subjectBioquímica
dc.subjectEhrlich ascites cells
dc.subjectFatty acid
dc.titleIn vitro conversion of saturated to monounsaturated fatty acid by Ehrlich ascites cells
dc.typeArticulo
dc.typeArticulo


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