Photothermal therapy with silver nanoplates in HeLa cells studied by <i>in situ</i> fluorescence microscopy

dc.creatorRivas Aiello, María Belén
dc.creatorAzcárate, Julio César
dc.creatorZelaya, Eugenia
dc.creatorGara, Pedro David
dc.creatorBosio, Gabriela Natalia
dc.creatorGensch, Thomas
dc.creatorMártire, Daniel Osvaldo
dc.date2021-02
dc.date2022-04-26T15:35:29Z
dc.date.accessioned2023-07-15T05:04:29Z
dc.date.available2023-07-15T05:04:29Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/135035
dc.identifierissn:2047-4849
dc.identifierissn:2047-4830
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7471988
dc.descriptionPhotothermal therapy (PTT) is a noninvasive treatment for cancer relying on the incorporation of NIR-light absorbing nanomaterials into cells, which upon illumination release heat causing thermally induced cell death. We prove that irradiation of aqueous suspensions of poly(vinylpyrrolidone)-coated silver nanoplates (PVPAgNP) or PVPAgNP in HeLa cells with red or NIR lasers causes a sizeable photothermal effect, which in cells can be visualized with the temperature sensing fluorophore Rhodamine B (RhB) using spinning disk confocal fluorescence microscopy or fluorescence lifetime imaging. Upon red-light irradiation of cells that were incubated with both, RhB and PVPAgNP at concentrations with no adverse effects on cell viability, a substantial heat release is detected. Initiation of cell death by photothermal effect is observed by positive signals of fluorescent markers for early and late apoptosis. Surprisingly, a new nanomaterial-assisted cell killing mode is operating when PVPAgNP-loaded HeLa cells are excited with moderate powers of fs-pulsed NIR light. Small roundish areas are generated with bright and fast (<1 ns) decaying emission, which expand fast and destroy the whole cell in seconds. This characteristic emission is assigned to efficient optical breakdown initiation around the strongly absorbing PVPAgNP leading to plasma formation that spreads fast through the cell.
dc.descriptionFacultad de Ciencias Exactas
dc.descriptionInstituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
dc.descriptionFacultad de Ingeniería
dc.descriptionCentro de Investigaciones Ópticas
dc.formatapplication/pdf
dc.format2608-2619
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by/3.0/
dc.rightsCreative Commons Attribution 3.0 Unported (CC BY 3.0)
dc.subjectQuímica
dc.subjectFísica
dc.subjectphotothermal therapy
dc.subjectsilver nanoplates
dc.subjectcell
dc.titlePhotothermal therapy with silver nanoplates in HeLa cells studied by <i>in situ</i> fluorescence microscopy
dc.typeArticulo
dc.typeArticulo


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