Cytotoxic and genotoxic effects induced by enrofloxacin-based antibiotic formulation Floxagen® in two experimental models of bovine cells in vitro : Peripheral lymphocytes and cumulus cells

dc.creatorAnchordoquy, Juan Patricio
dc.creatorAnchordoquy, Juan Mateo
dc.creatorNikoloff, Noelia
dc.creatorGambaro, Rocío Celeste
dc.creatorPadula, Gisel
dc.creatorFurnus, Cecilia Cristina
dc.creatorSeoane, Analía Isabel
dc.date2018-11-30
dc.date2022-09-19T18:26:26Z
dc.date.accessioned2023-07-15T04:55:43Z
dc.date.available2023-07-15T04:55:43Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/142354
dc.identifierissn:1614-7499
dc.identifierissn:0944-1344
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7471424
dc.descriptionThe in vitro effect of enrofloxacin (EFZ) was tested on two experimental somatic bovine cells in vitro: peripheral lymphocytes (PLs) and cumulus cells (CCs). The cytotoxicity and genotoxicity of this veterinary antibiotic were assessed using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assays, single-cell gel electrophoresis (SCGE) assay, and cytokinesis-block micronucleus cytome (CBMN cyt) assay. Cells were treated during 24 h, and three concentrations were tested (50 μg/mL, 100 μg/mL, 150 μg/mL). When EFZ was tested in PLs, the results demonstrated that the antibiotic was able to induce cell death and DNA damage with all concentrations. In addition, 50 μg/mL and 100 μg/mL EFZ increased frequencies of micronuclei (MNi). On the other hand, the highest EFZ concentration occasioned cellular cytotoxicity in CCs as evidenced by mitochondrial activity alterations. Nevertheless, EFZ was not able to induce DNA damage and MNi in CCs. These results represent the first experimental evidence of genotoxic and cytotoxic effects exerted by EFZ in bovine PLs and CCs.
dc.descriptionInstituto de Genética Veterinaria
dc.formatapplication/pdf
dc.format2998-3005
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by/4.0/
dc.rightsCreative Commons Attribution 4.0 International (CC BY 4.0)
dc.subjectVeterinaria
dc.subjectBovine cells
dc.subjectMTT
dc.subjectSCGE assay
dc.subjectCBMN cyt assay
dc.subjectEnrofloxacin
dc.titleCytotoxic and genotoxic effects induced by enrofloxacin-based antibiotic formulation Floxagen® in two experimental models of bovine cells in vitro : Peripheral lymphocytes and cumulus cells
dc.typeArticulo
dc.typeArticulo


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