| dc.creator | Pérez, Aurora | |
| dc.creator | Carvajal, Carol | |
| dc.creator | Trejo, Sebastián Alejandro | |
| dc.creator | Figuerero Torres, María José | |
| dc.creator | Martin, María Inés | |
| dc.creator | Lorenzo, José Carlos | |
| dc.creator | Natalucci, Claudia Luisa | |
| dc.creator | Hernández, Martha | |
| dc.date | 2010-05 | |
| dc.date | 2022-05-31T19:03:47Z | |
| dc.date.accessioned | 2023-07-15T04:50:07Z | |
| dc.date.available | 2023-07-15T04:50:07Z | |
| dc.identifier | http://sedici.unlp.edu.ar/handle/10915/137088 | |
| dc.identifier | issn:1572-3887 | |
| dc.identifier | issn:1573-4943 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/7471063 | |
| dc.description | Penduliflorain I, a new plant endopeptidase, was isolated and characterized from <i>Hohenbergia penduliflora</i>. Crude extract was obtained from stems. A partially purified enzyme preparation was obtained by ethanol precipitation. This preparation showed maximum activity between pH 7.5 and 8.5, was stable at ionic strength (20% decrease in proteolytic activity could be detected after 2 h in 0.4 M sodium chloride solution), and exhibited high thermal stability (inactivation required heating for 20 min at 75 °C). Inhibition and activation assays indicated the cysteine nature of the enzymatic preparation. Penduliflorain I was purified by anion exchange chromatography (Q-Sepharose HP) by FPLC system. Homogeneity was confirmed by mass spectroscopy. Molecular mass of the enzyme was 23 412.847 Da (MALDI-TOF–MS). Kinetic parameters were determined for PFLNA (Kₘ = 0.3227 mM and k<sub>cat</sub> = 4.27 s⁻¹). The N-terminal sequence (AVPQSIDWRDYGAVTTDKNQ) of isolated protease showed considerable similarity to other cysteine proteases obtained from stems or fruits of different <i>Bromeliaceae</i> species. | |
| dc.description | Centro de Investigación de Proteínas Vegetales | |
| dc.format | application/pdf | |
| dc.format | 225-233 | |
| dc.language | en | |
| dc.rights | http://creativecommons.org/licenses/by/4.0/ | |
| dc.rights | Creative Commons Attribution 4.0 International (CC BY 4.0) | |
| dc.subject | Ciencias Exactas | |
| dc.subject | Biología | |
| dc.subject | Bromeliaceae | |
| dc.subject | Hohenbergia penduliflora | |
| dc.subject | Chromatography purification | |
| dc.subject | Stems | |
| dc.subject | Thiol proteases | |
| dc.title | Penduliflorain I: A Cysteine Protease Isolated from <i>Hohenbergia penduliflora</i> (A.Rich.) Mez (<i>Bromeliaceae</i>) | |
| dc.type | Articulo | |
| dc.type | Articulo | |
