Inflammatory changes after cryosurgery-induced necrosis in human melanoma xenografted in nude mice

dc.creatorGazzaniga, Silvina
dc.creatorBravo, Alicia I.
dc.creatorGoldszmid, Silvana R.
dc.creatorMaschi, Fabricio Alejandro
dc.creatorMartinelli, Julio
dc.creatorMordoh, José
dc.creatorWainstok, Rosa
dc.date2001
dc.date2022-07-12T12:57:47Z
dc.date.accessioned2023-07-15T04:49:18Z
dc.date.available2023-07-15T04:49:18Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/139317
dc.identifierissn:0022-202x
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7471011
dc.descriptionThere is growing evidence that necrosis, instead of apoptosis, could act as a natural adjuvant, which could activate an immune response. In this work we have investigated if induction of tumor necrosis could trigger the affluence of inflammatory cells at the tumor site, and thus induce an immune response. For this purpose, a liquid N 2 spray was applied on human melanoma (IIB-MEL-J cell line) xenografted in nude mice and 24 h later some mice received intratumorally a single 500 U dose of recombinant murine granulocyte macrophage-colony-stimulating factor. 77–100% of the tumor mass underwent necrosis. Congestion, edema, and endothelial cell activation were the first noticeable events. A quick infiltrative response of polymorphonuclear leukocytes around the tumor was detected 24 h after liquid N 2 application, peaking at day 3. Massive macrophage recruitment was observed since day 3. An early intratumoral infiltration with inflammatory cells was only detected in the group that received recombinant murine granulocyte macrophage- colony-stimulating factor after necrosis induction by liquid N 2. Coexisting DEC 205- and F4/80-positive cells increased in number, and their localization was predominantly peritumoral after necrosis. Antibody response was only detected in the groups with tumor-induced necrosis. Our results suggest that cryosurgery-induced necrosis could be a useful model to analyze the interaction among necrosis, inflammation, and the generation of an immune response.
dc.descriptionFacultad de Ciencias Veterinarias
dc.formatapplication/pdf
dc.format664-671
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rightsCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.subjectVeterinaria
dc.subjectMedicina
dc.subjectcryosurgery
dc.subjectgranulocyte-monocyte colony-stimulating factor
dc.subjectinflammatory infiltrate
dc.subjectmelanoma
dc.titleInflammatory changes after cryosurgery-induced necrosis in human melanoma xenografted in nude mice
dc.typeArticulo
dc.typeArticulo


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