| dc.creator | Cerezo López, José María | |
| dc.creator | Lencina, F. | |
| dc.creator | Petruccelli, Silvana | |
| dc.creator | Marconi, Patricia Laura | |
| dc.creator | Álvarez, María Alejandra | |
| dc.date | 2010-06-29 | |
| dc.date | 2022-04-04T15:03:45Z | |
| dc.date.accessioned | 2023-07-15T04:39:47Z | |
| dc.date.available | 2023-07-15T04:39:47Z | |
| dc.identifier | http://sedici.unlp.edu.ar/handle/10915/133823 | |
| dc.identifier | issn:0167-6857 | |
| dc.identifier | issn:1573-5044 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/7470412 | |
| dc.description | We have established two transgenic cell suspension culture lines of <i>Nicotiana tabacum</i> that express the catalytic antibody 14D9 as a secretory product (sec-Ab) or as a KDEL-tagged product in the endoplasmic reticulum (Ab-KDEL), respectively. After 3 years of culture, the performance improved to a production level of 0.15 ± 0.03 μg ml<sup>−1</sup> on the seventh day of culture for the sec-Ab line and 0.48 ± 0.05 μg ml<sup>−1</sup> on the third day for Ab-KDEL line. Analysis of the effect of osmotic stress using mannitol (90 g l<sup>−1</sup>) as an osmolite revealed that there was a 12-fold increase in antibody yield (1.96 ± 0.20 μg ml<sup>−1</sup>) on the seventh day of culture in line sec-Ab and a fivefold increase (2.31 ± 0.18 μg ml<sup>−1</sup>) on the seventh day for line Ab-KDEL. The concentration of the antibody in the culture medium was not significant. Dimethyl sulfoxide used as a permeabilizing agent was not effective in increasing 14D9 yield, but it did cause distinctive cell damage at all concentrations tested. | |
| dc.description | Facultad de Ciencias Exactas | |
| dc.format | application/pdf | |
| dc.format | 307-314 | |
| dc.language | en | |
| dc.rights | http://creativecommons.org/licenses/by/4.0/ | |
| dc.rights | Creative Commons Attribution 4.0 International (CC BY 4.0) | |
| dc.subject | Bioquímica | |
| dc.subject | Molecular farming | |
| dc.subject | 14D9 | |
| dc.subject | In vitro plant cell cultures | |
| dc.subject | Recombinant proteins | |
| dc.title | Influence of the KDEL signal, DMSO and mannitol on the production of the recombinant antibody 14D9 by long-term Nicotiana tabacum cell suspension culture | |
| dc.type | Articulo | |
| dc.type | Articulo | |
