Argentina
| Articulo
Non-Muscle Myosin IIA (Myh9) is in the Nucleus of S-Phase Entering NT2-D1 Cells
| dc.creator | Naum-Onganía, Gabriela | |
| dc.creator | Rivera Pomar, Rolando Víctor | |
| dc.date | 2018-09-24 | |
| dc.date | 2021-11-05T17:21:59Z | |
| dc.date.accessioned | 2023-07-15T04:08:32Z | |
| dc.date.available | 2023-07-15T04:08:32Z | |
| dc.identifier | http://sedici.unlp.edu.ar/handle/10915/127843 | |
| dc.identifier | issn:1929-5634 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/7468409 | |
| dc.description | Non-muscle myosin IIA is a cytoplasmic protein that works in concert with F-actin to produce cell movement. The heavy chain of this protein is codified by the MYH9 gene. The presence of motor proteins as myosin or mono and F-actin and their role in transcription has recently been observed. Prep1–the transcription factor of HOXB genes– constitutes a dimer with Pbx1, which induces HOXB gene expression. Prep1 has been found purifying with β-actin and Myh9. HOXB transcription initiates when cells enter in S-phase, during which DNA duplication and transcription occur at the same time. Here, we have shown that Myh9 co-localizes with Prep1 in the nucleus and in the periphery of the nucleolus in S-phase NT2-D1 cells. Furthermore, we have shown that Myh9 purifies with Pbx1 from nuclear extracts of S-phase entering NT2-D1 cells –and not from cytoplasmic extracts. Taking into account these results, we conclude that Myh9 is in the nucleus of the S-phase entering NT2-D1 cells and might have a role in HOXB transcription. | |
| dc.description | Centro Regional de Estudios Genómicos | |
| dc.format | application/pdf | |
| dc.format | 59-66 | |
| dc.language | en | |
| dc.rights | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
| dc.rights | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
| dc.subject | Biología | |
| dc.subject | Myosin9 | |
| dc.subject | Hox genes | |
| dc.subject | NT2-D1 cells | |
| dc.subject | S-phase | |
| dc.title | Non-Muscle Myosin IIA (Myh9) is in the Nucleus of S-Phase Entering NT2-D1 Cells | |
| dc.type | Articulo | |
| dc.type | Articulo |