Unraveling the oral cancer lncRNAome: Identification of novel lncRNAs associated with malignant progression and HPV infection

dc.creatorNohata, Nijiro
dc.creatorAbba, Martín Carlos
dc.creatorGutkind, J. Silvio
dc.date2016
dc.date2021-11-18T16:13:25Z
dc.date.accessioned2023-07-15T04:06:02Z
dc.date.available2023-07-15T04:06:02Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/128359
dc.identifierissn:1879-0593
dc.identifierissn:1368-8375
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7468253
dc.descriptionObjectives The role of long non-coding RNA (lncRNA) expression in human head and neck squamous cell carcinoma (HNSCC) is still poorly understood. In this study, we aimed at establishing the onco-lncRNAome profiling of HNSCC and to identify lncRNAs correlating with prognosis and patient survival. Materials and methods The Atlas of Noncoding RNAs in Cancer (TANRIC) database was employed to retrieve the lncRNA expression information generated from The Cancer Genome Atlas (TCGA) HNSCC RNA-sequencing data. RNA-sequencing data from HNSCC cell lines were also considered for this study. Bioinformatics approaches, such as differential gene expression analysis, survival analysis, principal component analysis, and Co-LncRNA enrichment analysis were performed. Results Using TCGA HNSCC RNA-sequencing data from 426 HNSCC and 42 adjacent normal tissues, we found 728 lncRNA transcripts significantly and differentially expressed in HNSCC. Among the 728 lncRNAs, 55 lncRNAs were significantly associated with poor prognosis, such as overall survival and/or disease-free survival. Next, we found 140 lncRNA transcripts significantly and differentially expressed between Human Papilloma Virus (HPV) positive tumors and HPV negative tumors. Thirty lncRNA transcripts were differentially expressed between TP53 mutated and TP53 wild type tumors. Co-LncRNA analysis suggested that protein-coding genes that are co-expressed with these deregulated lncRNAs might be involved in cancer associated molecular events. With consideration of differential expression of lncRNAs in a HNSCC cell lines panel (n = 22), we found several lncRNAs that may represent potential targets for diagnosis, therapy and prevention of HNSCC. Conclusion LncRNAs profiling could provide novel insights into the potential mechanisms of HNSCC oncogenesis.
dc.descriptionCentro de Investigaciones Inmunológicas Básicas y Aplicadas
dc.formatapplication/pdf
dc.format58-66
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rightsCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.subjectCiencias Médicas
dc.subjectLong non-coding RNA
dc.subjectRNA-sequencing
dc.subjecthead and neck squamous cell carcinoma (HNSCC)
dc.subjecthuman papilloma virus (HPV)
dc.subjectTP53
dc.subjectTCGA
dc.titleUnraveling the oral cancer lncRNAome: Identification of novel lncRNAs associated with malignant progression and HPV infection
dc.typeArticulo
dc.typeArticulo


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