dc.creatorReid, Lara Olivia
dc.creatorCastaño, Carolina
dc.creatorDántola, María Laura
dc.creatorLhiaubet-Vallet, Virginie
dc.creatorMiranda, Miguel A.
dc.creatorMarin, M. Luisa
dc.creatorThomas, Andrés Héctor
dc.date2017
dc.date2021-11-16T13:35:17Z
dc.date.accessioned2023-07-15T04:05:34Z
dc.date.available2023-07-15T04:05:34Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/128209
dc.identifierissn:0143-7208
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7468224
dc.descriptionOxidative damage to proteins leads to a variety of modifications that are markers of pathogenesis. One of the most important modifications is the dityrosine (Tyr₂ ) cross-link, resulting from an oxidative covalent bond between two tyrosines (Tyr). An optimized methodology for preparation of pure Tyr₂ is important to investigate in detail its physicochemical properties and reactivity. Pterin (Ptr), the parent and unsubstituted compound of oxidized pterins, is able to photosensitize the cross-linking of free tyrosine (Tyr) and tyrosine residues of peptides and proteins through a photoinduced electron transfer mechanism. We have optimized a simple, one-step photocatalyzed formation of Tyr₂ , using Ptr as photocatalyst. Our procedure is carried out in aqueous solutions under UV-A radiation for few minutes. The purification of Tyr₂ is performed by reverse-phase chromatography. The obtained highly pure solution is used to fully characterize the Tyr₂ (exact mass and ¹H, ¹H- ¹H COSY; DEPT; HSQC and HMBC NMR experiments) and to deeper study its fluorescence properties.
dc.descriptionInstituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
dc.formatapplication/pdf
dc.format67-74
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rightsCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.subjectQuímica
dc.subjectDityrosine
dc.subjectOxidation
dc.subjectCross-linking
dc.subjectPterin
dc.subjectPhotocatalyzed synthesis
dc.subjectPhotoinduced electron transfer
dc.titleA novel synthetic approach to tyrosine dimers based on pterin photosensitization
dc.typeArticulo
dc.typePreprint


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