| dc.creator | Reid, Lara Olivia | |
| dc.creator | Castaño, Carolina | |
| dc.creator | Dántola, María Laura | |
| dc.creator | Lhiaubet-Vallet, Virginie | |
| dc.creator | Miranda, Miguel A. | |
| dc.creator | Marin, M. Luisa | |
| dc.creator | Thomas, Andrés Héctor | |
| dc.date | 2017 | |
| dc.date | 2021-11-16T13:35:17Z | |
| dc.date.accessioned | 2023-07-15T04:05:34Z | |
| dc.date.available | 2023-07-15T04:05:34Z | |
| dc.identifier | http://sedici.unlp.edu.ar/handle/10915/128209 | |
| dc.identifier | issn:0143-7208 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/7468224 | |
| dc.description | Oxidative damage to proteins leads to a variety of modifications that are markers of pathogenesis. One of the most important modifications is the dityrosine (Tyr₂ ) cross-link, resulting from an oxidative covalent bond between two tyrosines (Tyr). An optimized methodology for preparation of pure Tyr₂ is important to investigate in detail its physicochemical properties and reactivity. Pterin (Ptr), the parent and unsubstituted compound of oxidized pterins, is able to photosensitize the cross-linking of free tyrosine (Tyr) and tyrosine residues of peptides and proteins through a photoinduced electron transfer mechanism. We have optimized a simple, one-step photocatalyzed formation of Tyr₂ , using Ptr as photocatalyst. Our procedure is carried out in aqueous solutions under UV-A radiation for few minutes. The purification of Tyr₂ is performed by reverse-phase chromatography. The obtained highly pure solution is used to fully characterize the Tyr₂ (exact mass and ¹H, ¹H- ¹H COSY; DEPT; HSQC and HMBC NMR experiments) and to deeper study its fluorescence properties. | |
| dc.description | Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas | |
| dc.format | application/pdf | |
| dc.format | 67-74 | |
| dc.language | en | |
| dc.rights | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
| dc.rights | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
| dc.subject | Química | |
| dc.subject | Dityrosine | |
| dc.subject | Oxidation | |
| dc.subject | Cross-linking | |
| dc.subject | Pterin | |
| dc.subject | Photocatalyzed synthesis | |
| dc.subject | Photoinduced electron transfer | |
| dc.title | A novel synthetic approach to tyrosine dimers based on pterin photosensitization | |
| dc.type | Articulo | |
| dc.type | Preprint | |
