| dc.creator | Serena, María Soledad | |
| dc.creator | Geisler, Christoph | |
| dc.creator | Metz, Germán Ernesto | |
| dc.creator | Corva, Santiago Gerardo | |
| dc.creator | Mórtola, Eduardo Carlos | |
| dc.creator | Larsen, Alejandra Edith | |
| dc.creator | Jarvis, Donald L. | |
| dc.creator | Echeverría, María Gabriela | |
| dc.date | 2013-04-28 | |
| dc.date | 2021-12-01T17:43:08Z | |
| dc.date.accessioned | 2023-07-15T04:00:55Z | |
| dc.date.available | 2023-07-15T04:00:55Z | |
| dc.identifier | http://sedici.unlp.edu.ar/handle/10915/129004 | |
| dc.identifier | issn:1096-0279 | |
| dc.identifier | issn:1046-5928 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/7467924 | |
| dc.description | Suid Herpesvirus 1 (SHV-1) is the etiological agent of Aujeszky’s disease (AD), which affects swine herds worldwide and causes substantial economic losses due to animal mortality and lost productivity. In order to eradicate SHV-1, vaccination programs using viruses lacking the gene encoding glycoprotein E (gE) are ongoing in several countries. These eradication programs have generated a currently unmet demand for affordable and sensitive tests that can detect SHV-1 infection, yet distinguish between infected and vaccinated pigs. To meet this demand, we used the baculovirus-insect cell system to produce immunologically authentic full-length recombinant gE protein for use in a serum ELISA assay. As previous efforts to clone the gE gene had failed due to its extremely high GC-content (75% average), we used betaine as a PCR enhancer to facilitate amplification of the entire gE gene from the Argentinian CL15 strain of SHV-1. The cloned gE gene was expressed at high levels in recombinant baculovirus-infected insect cells and reacted strongly with sera from SHV-1 infected pigs. We used the recombinant gE protein to develop a local indirect ELISA test with sensitivity and specificity comparable to currently available commercial tests. Thus, recombinant gE produced in baculovirus-infected insect cells is a viable source of antigen for the detection of SHV-1 in ELISA tests. We also provide evidence supporting a potential application of this recombinant form of gE as a SHV-1 subunit vaccine. | |
| dc.description | Facultad de Ciencias Veterinarias | |
| dc.format | application/pdf | |
| dc.format | 1-8 | |
| dc.language | en | |
| dc.rights | http://creativecommons.org/licenses/by/4.0/ | |
| dc.rights | Creative Commons Attribution 4.0 International (CC BY 4.0) | |
| dc.subject | Ciencias Veterinarias | |
| dc.subject | Suid Herpesvirus 1 | |
| dc.subject | Baculovirus | |
| dc.subject | Insect cell | |
| dc.subject | Glycoprotein | |
| dc.subject | ELISA | |
| dc.subject | GC content | |
| dc.title | Expression and purification of Suid Herpesvirus-1 glycoprotein E in the baculovirus system and its use to diagnose Aujeszky's disease in infected pigs | |
| dc.type | Articulo | |
| dc.type | Articulo | |
