| dc.creator | Laino, Aldana | |
| dc.creator | Lavarías, Sabrina María Luisa | |
| dc.creator | Suárez, Gustavo | |
| dc.creator | Lino, Agustina Berenice | |
| dc.creator | Cunningham, Mónica Liliana | |
| dc.date | 2015-10 | |
| dc.date | 2021-11-03T18:33:16Z | |
| dc.date.accessioned | 2023-07-15T03:59:14Z | |
| dc.date.available | 2023-07-15T03:59:14Z | |
| dc.identifier | http://sedici.unlp.edu.ar/handle/10915/127717 | |
| dc.identifier | issn:1932-5223 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/7467816 | |
| dc.description | Hemocyanin of the spider <i>Polybetes pythagoricus</i>, in addition to its typical role as an oxygen transporter, also exhibits a phenoloxidase activity induced by micellar concentrations of SDS. In the present work, we found the kinetic parameters K<sub>m</sub> and V<sub>max</sub> of <i>Polybetes pythagoricus</i> hemocyanin (<i>Pp</i>Hc) PO activity to be 0.407mM and 0.081 μmolmin⁻¹mg protein⁻¹, respectively. Dopamine was used as the substrate with SDS at a final concentration of 10mM and a 30-min incubation at 25°C. Conformational changes in Hc associated with the SDS treatment were analyzed using far-UV circular dichroism, intrinsic fluorescence and absorption spectroscopy. The secondary and tertiary structural changes of <i>Pp</i>Hc induced by SDS led to increases in α-helical content and tryptophan fluorescence intensity. A reduction in the absorption spectrum at 340 nm in the presence of SDS was also observed. These results suggest that the SDS-induced PO activity of <i>Pp</i>Hc can be ascribed to conformational changes in the local environment of the typer-3 copper active site. | |
| dc.description | Facultad de Ciencias Médicas | |
| dc.description | Instituto de Investigaciones Bioquímicas de La Plata | |
| dc.description | Facultad de Ciencias Exactas | |
| dc.description | Laboratorio de Investigación en Osteopatías y Metabolismo Mineral | |
| dc.description | Facultad de Ciencias Naturales y Museo | |
| dc.description | Instituto de Limnología "Dr. Raúl A. Ringuelet" | |
| dc.format | application/pdf | |
| dc.format | 547-555 | |
| dc.language | en | |
| dc.rights | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
| dc.rights | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
| dc.subject | Ciencias Exactas | |
| dc.subject | Ciencias Naturales | |
| dc.subject | Polybetes pythagoricus | |
| dc.subject | Hemocyanin | |
| dc.subject | Phenoloxidase Activity | |
| dc.title | Characterization of Phenoloxidase Activity From Spider <i>Polybetes pythagoricus</i> Hemocyanin | |
| dc.type | Articulo | |
| dc.type | Preprint | |
