dc.creatorDa Cruz Cabral, Lucía Mariana
dc.creatorPiccinali, Romina Valeria
dc.creatorLanteri, Analía Alicia
dc.creatorConfalonieri, Viviana A.
dc.creatorRodriguero, Marcela Silvina
dc.date2020-07-17
dc.date2021-09-28T13:29:43Z
dc.date.accessioned2023-07-15T03:16:02Z
dc.date.available2023-07-15T03:16:02Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/125726
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7465061
dc.descriptionThe intracellular bacteria <i>Wolbachia pipientis</i> can manipulate host reproduction to enhance their vertical transmission. It has been reported an association between parthenogenesis and <i>Wolbachia</i> infection in weevils from the tribe Naupactini. A curing experiment suggested that a threshold density of <i>Wolbachia</i> is required for parthenogenesis to occur. The aim of this study was to analyze <i>Wolbachia</i> infection status in the bisexual species <i>Naupactus xanthographus</i> and <i>Naupactus dissimulator</i>. <i>Wolbachia</i> infection was detected in both species from some geographic locations, not being fixed. In all positive cases, faint PCR bands were observed. Quantification through real time PCR confirmed that <i>Wolbachia</i> loads in bisexual species were significantly lower than in parthenogenetic ones; this strengthens the hypothesis of a threshold level. Strain typing showed that both species carry <i>w</i>Nau1, the most frequent in parthenogenetic Naupactini weevils. These infections seem to be recently acquired by horizontal transfer. <i>Wolbachia</i> was located throughout the whole body, which reinforce the idea of recent transmission. Moreover, we demonstrated that this strain carries the WO phage. Finally, the analysis of eubacterial 16S <i>rRNA</i> gene showed intense PCR bands for both bisexual species, suggesting –the presence of additional bacteria. Interspecific competition might explain why the parthenogenetic phenotype is not triggered.
dc.descriptionFacultad de Ciencias Naturales y Museo
dc.formatapplication/pdf
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by/4.0/
dc.rightsCreative Commons Attribution 4.0 International (CC BY 4.0)
dc.subjectBiología
dc.subjectWolbachia
dc.subjectNaupactini
dc.subjectWeevils
dc.subjectThelytokous Parthenogenesis
dc.subjectWolbachia Titers
dc.subjectInteracting Microbiota
dc.titleParthenogenesis in weevils of the tribe Naupactini (Coleoptera, Curculionidae) : A Wolbachia-density dependent trait?
dc.typeArticulo
dc.typePreprint


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