| dc.creator | Da Cruz Cabral, Lucía Mariana | |
| dc.creator | Piccinali, Romina Valeria | |
| dc.creator | Lanteri, Analía Alicia | |
| dc.creator | Confalonieri, Viviana A. | |
| dc.creator | Rodriguero, Marcela Silvina | |
| dc.date | 2020-07-17 | |
| dc.date | 2021-09-28T13:29:43Z | |
| dc.date.accessioned | 2023-07-15T03:16:02Z | |
| dc.date.available | 2023-07-15T03:16:02Z | |
| dc.identifier | http://sedici.unlp.edu.ar/handle/10915/125726 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/7465061 | |
| dc.description | The intracellular bacteria <i>Wolbachia pipientis</i> can manipulate host reproduction to enhance their vertical transmission. It has been reported an association between parthenogenesis and <i>Wolbachia</i> infection in weevils from the tribe Naupactini. A curing experiment suggested that a threshold density of <i>Wolbachia</i> is required for parthenogenesis to occur. The aim of this study was to analyze <i>Wolbachia</i> infection status in the bisexual species <i>Naupactus xanthographus</i> and <i>Naupactus dissimulator</i>. <i>Wolbachia</i> infection was detected in both species from some geographic locations, not being fixed. In all positive cases, faint PCR bands were observed. Quantification through real time PCR confirmed that <i>Wolbachia</i> loads in bisexual species were significantly lower than in parthenogenetic ones; this strengthens the hypothesis of a threshold level. Strain typing showed that both species carry <i>w</i>Nau1, the most frequent in parthenogenetic Naupactini weevils. These infections seem to be recently acquired by horizontal transfer. <i>Wolbachia</i> was located throughout the whole body, which reinforce the idea of recent transmission. Moreover, we demonstrated that this strain carries the WO phage. Finally, the analysis of eubacterial 16S <i>rRNA</i> gene showed intense PCR bands for both bisexual species, suggesting –the presence of additional bacteria. Interspecific competition might explain why the parthenogenetic phenotype is not triggered. | |
| dc.description | Facultad de Ciencias Naturales y Museo | |
| dc.format | application/pdf | |
| dc.language | en | |
| dc.rights | http://creativecommons.org/licenses/by/4.0/ | |
| dc.rights | Creative Commons Attribution 4.0 International (CC BY 4.0) | |
| dc.subject | Biología | |
| dc.subject | Wolbachia | |
| dc.subject | Naupactini | |
| dc.subject | Weevils | |
| dc.subject | Thelytokous Parthenogenesis | |
| dc.subject | Wolbachia Titers | |
| dc.subject | Interacting Microbiota | |
| dc.title | Parthenogenesis in weevils of the tribe Naupactini (Coleoptera, Curculionidae) : A Wolbachia-density dependent trait? | |
| dc.type | Articulo | |
| dc.type | Preprint | |
