Nanostructured fumarate copolymer-chitosan crosslinked scaffold: An in vitro osteochondrogenesis regeneration study

dc.creatorLastra, María Laura
dc.creatorMolinuevo, María Silvina
dc.creatorBlaszczyk Lezak, Iwona
dc.creatorMijangos, Carmen
dc.creatorCortizo, María Susana
dc.date2017-11-20
dc.date2021-09-08T16:17:50Z
dc.date.accessioned2023-07-15T03:13:33Z
dc.date.available2023-07-15T03:13:33Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/124415
dc.identifierissn:1552-4965
dc.identifierissn:1549-3296
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7464906
dc.descriptionIn the tissue engineering field, the design of the scaffold inspired on the natural occurring tissue is of vital importance. Ideally, the scaffold surface must promote cell growth and differentiation, while promote angiogenesis in the in vivo implant of the scaffold. On the other hand, the material selection must be biocompatible and the degradation times should meet tissue reparation times. In the present work, we developed a nanofibrous scaffold based on chitosan crosslinked with diisopropylfumarate-vinyl acetate copolymer using anodized aluminum oxide (AAO) templates. We have previously demonstrated its biocompatibility properties with low cytotoxicity and proper degradation times. Now, we extended our studies to demonstrate that it can be successfully nanostructured using the AAO templates methodology, obtaining a nanorod-like scaffold with a diameter comparable to those of collagen fibers of the bone matrix (170 and 300 nm). The nanorods obtained presented a very homogeneous pattern in diameter and length, and supports cell attachment and growth. We also found that both osteoblastic and chondroblastic matrix production were promoted on bone marrow progenitor cells and primary condrocytes growing on the scaffolds, respectively. In addition, the nanostructured scaffold presented no cytotoxicity as it was evaluated using a model of macrophages on culture.
dc.descriptionLaboratorio de Investigación en Osteopatías y Metabolismo Mineral
dc.descriptionInstituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
dc.formatapplication/pdf
dc.format570-579
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rightsCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.subjectCiencias Exactas
dc.subjectCytotoxicity
dc.subjectBiomedical engineering
dc.subjectRegeneration (biology)
dc.subjectMaterials science
dc.subjectBiocompatibility
dc.subjectChitosan
dc.subjectBone regeneration
dc.subjectTissue engineering
dc.subjectScaffold
dc.subjectNanofiber
dc.subjectCartilage regeneration
dc.subjectNanostructured biomaterials
dc.subjectPolyfumarate
dc.titleNanostructured fumarate copolymer-chitosan crosslinked scaffold: An in vitro osteochondrogenesis regeneration study
dc.typeArticulo
dc.typePreprint


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