Enzootic bovine leukosis: development of an indirect enzyme linked immunosorbent assay (I-ELISA) in seroepidemiological studies

dc.creatorGonzález, Ester Teresa
dc.creatorBonzo, Estela Beatriz
dc.creatorEcheverría, María Gabriela
dc.creatorLicursi, María
dc.creatorEtcheverrigaray, María Elisa
dc.date1999
dc.date2021-03-16T18:46:37Z
dc.date.accessioned2023-07-15T00:49:23Z
dc.date.available2023-07-15T00:49:23Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/115089
dc.identifierissn:0001-3714
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7455943
dc.descriptionBovine Leukemia Virus (BLV) is the etiologic agent of Enzootic Bovine Leukosis, a retrovirus exogenous to the bovine species. Once infected, there is no detectable viraemia but instead there is a strong and persistent immunological response to BLV structural proteins, essentially the gp51 envelope glycoprotein and the mayor core protein p24. We describe the test procedure of an indirect ELISA (I-ELISA) using polyclonal reagents for the detection of antibodies to BLV. For comparison, the sera were simultaneously tested by agar gel immunodiffussion (AGID) test, which is currently used as diagnostic standard for BLV infection. The antigen applied does not require a high degree of purification and the data from the analysis of the negative sera showed that the establishment of a cut-off level corresponding to 3 times the standard deviation (SD) above the mean for the negative control set of sera provided acceptable specificity, reducing the risk of false positives results. A comparison of the results obtained by AGID test and I-ELISA showed that considering a total of 465 serum samples, all of the 234 samples (50%) that were positive by AGID were positive to the I-ELISA. Of 225 serum samples which yielded negative results in the AGID test, 69 (15%) were found to be positive by the I-ELISA and 156 (33%) were negative by both techniques. Few sera (2%) that were non-specific by AGID were defined as negative or positive by I-ELISA.
dc.descriptionFacultad de Ciencias Veterinarias
dc.formatapplication/pdf
dc.format37-42
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc/4.0/
dc.rightsCreative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
dc.subjectCiencias Veterinarias
dc.subjectBovine Leukaemia
dc.subjectindirect ELISA
dc.subjectseroepidemiology
dc.titleEnzootic bovine leukosis: development of an indirect enzyme linked immunosorbent assay (I-ELISA) in seroepidemiological studies
dc.typeArticulo
dc.typeArticulo


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