<i>Citrus psorosis</i> and <i>Mirafiori lettuce big-vein</i> ophiovirus coat proteins localize to the cytoplasm and self interact in vivo

dc.creatorPeña, Eduardo José
dc.creatorRobles Luna, Gabriel
dc.creatorZanek, María Cecilia
dc.creatorBorniego, María Belén
dc.creatorReyes, Carina Andrea
dc.creatorHeinlein, Manfred
dc.creatorGarcía, María Laura
dc.date2012
dc.date2021-02-17T18:36:26Z
dc.date.accessioned2023-07-15T00:28:35Z
dc.date.available2023-07-15T00:28:35Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/112878
dc.identifierissn:0168-1702
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7454637
dc.descriptionCitrus psorosis (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV) belong to the family Ophioviridae, plant viruses with filamentous nucleocapsids and segmented genomes of negative polarity, causing the worldwide distributed citrus psorosis and lettuce big-vein diseases, respectively. To gain insight into the replication cycle of these viruses, the subcellular localization of the viral coat proteins (CP) was studied. Immunoblot analysis of fractionated extracts derived from natural and experimental infected hosts indicated that the CP of CPsV occurs in the soluble cytoplasmic fraction. The cytoplasmic localization of this protein was confirmed by confocal microscopy of fluorescent protein (FP)-tagged CP following its expression in either CPsV-infected and healthy Citrus sinensis plants or in Nicotiana benthamiana plants. The same localization was observed for FP-tagged CP of MiLBVV. The CPs of CPsV and MiLBBV can undergo homologous and heterologous interactions as revealed by fluorescent lifetime imaging microscopy and co-immunoprecipitation analysis. A putative leucine zipper motif that is conserved among ophiovirus CP sequences may account for these interactions.
dc.descriptionInstituto de Biotecnologia y Biologia Molecular
dc.formatapplication/pdf
dc.format34-43
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rightsCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.subjectBiología
dc.subjectCitrus psorosis virus
dc.subjectMirafiori lettuce big-vein virus
dc.subjectOphioviridae
dc.subjectCoat protein
dc.subjectProtein interaction
dc.subjectFRET
dc.subjectFLIM
dc.subjectImmunoprecipitation
dc.subjectSubcellular localization
dc.title<i>Citrus psorosis</i> and <i>Mirafiori lettuce big-vein</i> ophiovirus coat proteins localize to the cytoplasm and self interact in vivo
dc.typeArticulo
dc.typeArticulo


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