| dc.creator | Martínez, Carolina | |
| dc.creator | Petruccelli, Silvana | |
| dc.creator | Giulietti, Ana María | |
| dc.creator | Alvarez, María Alejandra | |
| dc.date | 2005 | |
| dc.date | 2020-11-09T17:16:56Z | |
| dc.date.accessioned | 2023-07-14T23:16:01Z | |
| dc.date.available | 2023-07-14T23:16:01Z | |
| dc.identifier | http://sedici.unlp.edu.ar/handle/10915/108597 | |
| dc.identifier | http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v8n2-10 | |
| dc.identifier | issn:0717-3458 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/7449987 | |
| dc.description | Nicotiana tabacum hairy roots that express the antibody 14D9 were established. The 14D9 antibody yield obtained after 20 days of culture was 5.95 μg 14D9ml-1. The addition of the reticulum endoplasmic retention sequence KDEL demonstrated a positive effect over the intracellular 14D9 amounts with a yield increase up to 20.82 μg ml-1. DMSO increased the antibody amount in the biomass from 20.00 to 64.03 μg ml-1 while PVP (at 1.5 gl-1) and gelatine (at 5.0 gl-1) increased total 14D9 amounts in the culture medium to 25 μg and 14 μg respectively. | |
| dc.description | Centro de Investigación y Desarrollo en Criotecnología de Alimentos | |
| dc.description | Universidad de Buenos Aires | |
| dc.format | application/pdf | |
| dc.format | 170-176 | |
| dc.language | en | |
| dc.rights | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.rights | Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) | |
| dc.subject | Bioquímica | |
| dc.subject | Catalytic antibodies | |
| dc.subject | In vitro cultures | |
| dc.subject | Molecular farming | |
| dc.subject | Recombinant antibodies | |
| dc.subject | Tobacco | |
| dc.title | Expression of the antibody 14D9 in <i>Nicotiana tabacum</i> hairy roots | |
| dc.type | Articulo | |
| dc.type | Articulo | |
