dc.creatorSisti, Federico
dc.creatorHa, Dae-Gon
dc.creatorO'Toole, George A.
dc.creatorHozbor, Daniela Flavia
dc.creatorFernández, Julieta
dc.date2013
dc.date2020-10-30T13:05:28Z
dc.date.accessioned2023-07-14T22:46:43Z
dc.date.available2023-07-14T22:46:43Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/107998
dc.identifierhttp://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC4085988&blobtype=pdf
dc.identifierissn:1465-2080
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7448170
dc.descriptionThe signalling molecule bis-(39–59)-cyclic-dimeric guanosine monophosphate (c-di-GMP) is a central regulator of diverse cellular functions, including motility, biofilm formation, cell cycle progression and virulence, in bacteria. Multiple diguanylate cyclase and phosphodiesterasedomain-containing proteins (GGDEF and EAL/HD-GYP, respectively) modulate the levels of the second messenger c-di-GMP to transmit signals and obtain such specific cellular responses. In the genus Bordetella this c-di-GMP network is poorly studied. In this work, we evaluated the expression of two phenotypes in Bordetella bronchiseptica regulated by c-di-GMP, biofilm formation and motility, under the influence of ectopic expression of Pseudomonas aeruginosa proteins with EAL or GGDEF domains that regulates the c-di-GMP level. In agreement with previous reports for other bacteria, we observed that B. bronchiseptica is able to form biofilm and reduce its motility only when GGDEF domain protein is expressed. Moreover we identify a GGDEF domain protein (BB3576) with diguanylate cyclase activity that participates in motility and biofilm regulation in B. bronchiseptica. These results demonstrate for the first time, to our knowledge, the presence of c-di-GMP regulatory signalling in B. bronchiseptica.
dc.descriptionInstituto de Biotecnologia y Biologia Molecular
dc.formatapplication/pdf
dc.format869-879
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rightsCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.subjectCiencias Exactas
dc.subjectBordetella bronchiseptica
dc.titleCyclic-di-GMP signalling regulates motility and biofilm formation in <i>Bordetella bronchiseptica</i>
dc.typeArticulo
dc.typeArticulo


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