Bordetella bronchiseptica Diguanylate Cyclase BdcA Regulates Motility and Is Important for the Establishment of Respiratory Infection in Mice

dc.creatorBelhart, Keila
dc.creatorGutierrez, María de la Paz
dc.creatorZacca, Federico
dc.creatorAmbrosis, Nicolás
dc.creatorCartelle Gestal, Monica
dc.creatorTaylor, Dawn
dc.creatorDahlstrom, Kurt M.
dc.creatorHarvill, Eric T.
dc.creatorO'Toole, George A.
dc.creatorSisti, Federico
dc.creatorFernández, Julieta
dc.date2019
dc.date2020-10-19T17:00:58Z
dc.date.accessioned2023-07-14T22:44:23Z
dc.date.available2023-07-14T22:44:23Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/107283
dc.identifierhttp://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6689298&blobtype=pdf
dc.identifierissn:1098-5530
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7448026
dc.descriptionBacteria can be motile and planktonic or, alternatively, sessile and participating in the biofilm mode of growth. The transition between these lifestyles can be regulated by a second messenger, cyclic dimeric GMP (c-di-GMP). High intracellular c-diGMP concentration correlates with biofilm formation and motility inhibition in most bacteria, including Bordetella bronchiseptica, which causes respiratory tract infections in mammals and forms biofilms in infected mice. We previously described the diguanylate cyclase BdcA as involved in c-di-GMP synthesis and motility regulation in B. bronchiseptica; here, we further describe the mechanism whereby BdcA is able to regulate motility and biofilm formation. Amino acid replacement of GGDEF with GGAAF in BdcA is consistent with the conclusion that diguanylate cyclase activity is necessary for biofilm formation and motility regulation, although we were unable to confirm the stability of the mutant protein. In the absence of the bdcA gene, B. bronchiseptica showed enhanced motility, strengthening the hypothesis that BdcA regulates motility in B. bronchiseptica. We showed that c-di-GMP-mediated motility inhibition involved regulation of flagellin expression, as high c-di-GMP levels achieved by expressing BdcA significantly reduced the level of flagellin protein. We also demonstrated that protein BB2109 is necessary for BdcA activity, motility inhibition, and biofilm formation. Finally, absence of the bdcA gene affected bacterial infection, implicating BdcA-regulated functions as important for bacterium-host interactions. This work supports the role of c-di-GMP in biofilm formation and motility regulation in B. bronchiseptica, as well as its impact on pathogenesis.
dc.descriptionInstituto de Biotecnologia y Biologia Molecular
dc.formatapplication/pdf
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rightsCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.subjectBiología
dc.subjectBiofilm
dc.subjectBordetella
dc.subjectMotility
dc.subjectc-di-GMP
dc.titleBordetella bronchiseptica Diguanylate Cyclase BdcA Regulates Motility and Is Important for the Establishment of Respiratory Infection in Mice
dc.typeArticulo
dc.typeArticulo


Este ítem pertenece a la siguiente institución