Comparative Analysis of the Biosimilar and Innovative G-CSF Modulated Pathways on Umbilical Cord Blood-Derived Mononuclear Cells

dc.creatorAvila-Portillo, L. M.
dc.creatorAristizabal, F.
dc.creatorPerdomo, S.
dc.creatorRiveros, A.
dc.creatorOspino, B.
dc.creatorAvila, J. P.
dc.creatorButti, Matías A.
dc.creatorAbba, Martín Carlos
dc.date2020
dc.date2020-10-28T13:34:40Z
dc.date.accessioned2023-07-14T22:43:59Z
dc.date.available2023-07-14T22:43:59Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/107843
dc.identifierhttp://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7088127&blobtype=pdf
dc.identifierissn:1177-9322
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7448001
dc.descriptionBiosimilars of granulocyte colony-stimulating factor (G-CSF) have been routinely introduced into clinical practice. However, not functional genomics characterization has been performed yet in comparison with the innovator G-CSF. This study aimed to evaluate the transcriptomic changes in an in vitro model of umbilical cord blood cells (UBC) exposed to G-CSF for the identification of their modulated pathways. Umbilical cord blood cells–derived mononuclear cells (MNCs) were treated with biosimilar and innovator G-CSF for further gene expression profiling analysis using a microarray-based platform. Comparative analysis of biosimilar and innovator G-CSF gene expression signatures allowed us to identify the most commonly modulated pathways by both drugs. In brief, we observed predominantly upmodulation of transcripts related to PI3K-Akt, NF-kappaB, and tumor necrosis factor (TNF) signaling pathways as well as transcripts related to negative regulation of apoptotic process among others. In addition, hematopoietic colony-forming cell assays corroborate the G-CSF phenotypic effects over UBC-derived MNCs. In conclusion, our study suggests that G-CSF impacts UBC-derived cells through the modulation of several signaling pathways associated with cell survival, migration, and proliferation. The concordance observed between biosimilar and innovator G-CSF emphasizes their similarity in regards to their specificity and biological responses.
dc.descriptionCentro de Investigaciones Inmunológicas Básicas y Aplicadas
dc.formatapplication/pdf
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc/4.0/
dc.rightsCreative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
dc.subjectBiología
dc.subjectG-CSF
dc.subjectInnovator
dc.subjectBiosimilar
dc.subjectUmbilical cord blood
dc.subjectTranscriptomics
dc.titleComparative Analysis of the Biosimilar and Innovative G-CSF Modulated Pathways on Umbilical Cord Blood-Derived Mononuclear Cells
dc.typeArticulo
dc.typeArticulo


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