Outer-Membrane-Vesicle-Associated O Antigen, a Crucial Component for Protecting Against Bordetella parapertussis Infection

dc.creatorBottero, Daniela
dc.creatorZurita, María Eugenia
dc.creatorGaillard, María Emilia
dc.creatorCarriquiriborde, Francisco Pablo
dc.creatorAispuro, Pablo Martín
dc.creatorElizagaray, Maia Lina
dc.creatorBartel, Erika Belén
dc.creatorCastuma, Celina Elisabet
dc.creatorHozbor, Daniela Flavia
dc.date2018
dc.date2020-09-14T16:57:51Z
dc.date.accessioned2023-07-14T22:00:14Z
dc.date.available2023-07-14T22:00:14Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/104549
dc.identifierhttp://hdl.handle.net/11336/96469
dc.identifierhttps://www.frontiersin.org/articles/10.3389/fimmu.2018.02501/full
dc.identifierissn:1664-3224
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7445233
dc.description<i>Bordetella parapertussis</i> is a respiratory-disease pathogen producing symptomatology similar to that of pertussis but of underestimated incidence and with no specific vaccine existing. We recently designed a vaccine candidate from <i>B. parapertussis</i> outer-membrane vesicles (OMVs) that proved to be safe and protective in a murine-infection model. Based on protection recently reported for the <i>B. parapertussis</i> O antigen in aqueous solution, we assessed here whether the <i>B. parapertussis</i> O-antigen-containing lipopolysaccharide (BppLPS-O⁺) embedded in the membranes, as present in <i>B. parapertussis</i>-derived OMVs (OMVs(Bpp-LPS-O⁺)), was the component responsible for that previously observed protection by OMVs. By performing a comparative study with OMVs from a human strain with undetectable O antigen (OMVs(Bpp-LPS-O⁻)), we demonstrated that the OMVs(Bpp-LPS-O⁺), but not the OMVs(Bpp-LPS-O⁻), protected mice against sublethal <i>B. parapertussis</i> infections. Indeed, the <i>B. parapertussis</i> loads were significantly reduced in the lungs of OMVs(Bpp-LPS-O⁺) -vaccinated animals, with the CFUs recovered being decreased by 4 log units below those detected in the non-immunized animals or in the animals treated with the OMVs(Bpp-LPS-O⁻), (p < 0.001). We detected that the OMVs(Bpp-LPS-O⁺) induced IgG antibodies against <i>B. parapertussis</i> whole-cell lysates, which immunocomponents recognized, among others, the O antigen and accordingly conferred protection against <i>B. parapertussis</i> infection, as observed in <i>in-vivo</i>-passive-transfer experiments. Of interest was that the OMVs(Bpp-LPS-O⁺) -generated sera had opsonophagocytic and bactericidal capabilities that were not detected with the OMVs(Bpp-LPS-O⁻)-induced sera, suggesting that those activities were involved in the clearance of <i>B. parapertussis</i>. Though stimulation of cultured spleen cells from immunized mice with formulations containing the O antigen resulted in gamma interferon (IFN-γ) and interleukin-17 production, spleen cells from OMVs(Bpp-LPS-O⁺) -immunized mice did not significantly contribute to the observed protection against <i>B. parapertussis</i> infection. The protective capability of the <i>B. parapertussis</i> O antigen was also detected in formulations containing both the OMVs derived from B. pertussis and purified BppLPS-O⁺. This combined formulation protected mice against B. pertussis along with <i>B. parapertussis</i>.
dc.descriptionFacultad de Ciencias Exactas
dc.descriptionInstituto de Biotecnologia y Biologia Molecular
dc.descriptionInstituto de Estudios Inmunológicos y Fisiopatológicos
dc.formatapplication/pdf
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by/4.0/
dc.rightsCreative Commons Attribution 4.0 International (CC BY 4.0)
dc.subjectCiencias Exactas
dc.subjectBiología
dc.subjectBordetella parapertussis
dc.subjectlipopolysacharides
dc.subjectO-antigen
dc.subjectouter-membrane vesicles
dc.subjectprotection
dc.titleOuter-Membrane-Vesicle-Associated O Antigen, a Crucial Component for Protecting Against Bordetella parapertussis Infection
dc.typeArticulo
dc.typeArticulo


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